Liver Disease and HPLC Quantification of Disialotransferrin for Heavy Alcohol Use: A Case Series
Article first published online: 26 OCT 2010
Copyright © 2010 by the Research Society on Alcoholism
Alcoholism: Clinical and Experimental Research
Volume 34, Issue 11, pages 1956–1960, November 2010
How to Cite
Stewart, S. H., Comte-Walters, S., Bowen, E. and Anton, R. F. (2010), Liver Disease and HPLC Quantification of Disialotransferrin for Heavy Alcohol Use: A Case Series. Alcoholism: Clinical and Experimental Research, 34: 1956–1960. doi: 10.1111/j.1530-0277.2010.01285.x
- Issue published online: 26 OCT 2010
- Article first published online: 26 OCT 2010
- Received for publication March 17, 2010; accepted May 19, 2010.
- Liver Disease;
- Carbohydrate-Deficient Transferrin
Background: It had previously been suggested that individuals with cirrhosis may have a pattern of transferrin glycosylation that interferes with the interpretation of carbohydrate-deficient transferrin (CDT) testing for heavy alcohol use. The goal of this case series was to evaluate the prevalence of liver disease among individuals with poor resolution of transferrin glycoforms by high performance liquid chromatography.
Methods: We reviewed the electronic medical records of 35 consecutive patients with poor chromatographic resolution of disialotransferrin from trisialotransferrin and recorded information on diagnosed liver disease, liver function testing, and other factors.
Results: Thirty of the 35 subjects with poor chromatographic resolution of the transferrin glycoforms had sufficient data in the medical record for some estimation of liver function. Of these 30 subjects, 25 had previously diagnosed liver pathology. Of the remaining 5 subjects, 2 had liver imaging results suggestive of benign tumor; the remaining 3 had mildly elevated bilirubin and aminotransferase activity, and low albumin.
Conclusions: Liver abnormalities, but not necessarily cirrhosis, are common in individuals with poor chromatographic separation of transferrin glycoforms, which might lead to false-positive results on CDT testing. However, the chromatographic-based assay can detect this issue, minimizing the reporting of false positives, but not necessarily assisting in valid detection of heavy drinking.