Miguel Marcos and Isabel Pastor contributed equally to this work.
Cannabinoid Receptor 1 Gene is Associated with Alcohol Dependence
Version of Record online: 15 NOV 2011
Copyright © 2011 by the Research Society on Alcoholism
Alcoholism: Clinical and Experimental Research
Volume 36, Issue 2, pages 267–271, February 2012
How to Cite
Marcos, M., Pastor, I., de la Calle, C., Barrio-Real, L., Laso, F.-J. and González-Sarmiento, R. (2012), Cannabinoid Receptor 1 Gene is Associated with Alcohol Dependence. Alcoholism: Clinical and Experimental Research, 36: 267–271. doi: 10.1111/j.1530-0277.2011.01623.x
- Issue online: 27 JAN 2012
- Version of Record online: 15 NOV 2011
- Received for publication January 3, 2011; accepted June 6, 2011.
- Alcohol Abuse;
- Alcohol Dependence;
- Cannabinoid Receptor CB1;
Background: Alcohol dependence (AD) vulnerability is determined by a complex array of genetic factors. Given the potential role of endocannabinoid system in AD, polymorphisms within cannabinoid receptor 1 gene (CNR1) have been potentially associated with susceptibility to this disease. We thus aimed to examine the relationship between 3 allelic variants of CNR1 (rs6454674, rs1049353, and rs806368) and AD.
Methods: Genotyping of the aforementioned polymorphisms was carried out by PCR in 298 male alcoholics (187 of them with AD) and 155 healthy controls. Single-marker, haplotype, and interaction analysis were performed to analyze the influence of CNR1 gene on AD susceptibility.
Results: We found an association between CNR1 gene and AD after haplotype analysis. Alcoholic patients with TGT haplotype (corresponding to rs6454674-rs1049353-rs806368 polymorphisms in this order) were less prone to have AD (p = 0.017). Besides, alcoholics with a G/T substitution of the first marker (GGT haplotype) or a C/T substitution of the third marker (TGC haplotype) were more likely to develop AD (p = 0.006 and 0.004, respectively) and an interaction was found between the G allele of rs6454674 single nucleotide polymorphism (SNP) and the C allele of rs806368 SNP (p = 0.009).
Conclusions: Our findings support previously reported associations of CNR1 with dependence to alcohol and other substances and emphasizes the relevance of endocannabinoid system in AD.