The Impact of Alcohol on BCG-Induced Immunity Against Mycobacterium tuberculosis

Authors

  • Elizabeth Porretta,

    1. From the Pulmonary/Critical Care Medicine(EP, KIH, XST, CMM), Louisiana State University Health Sciences Center, New Orleans, Louisiana; and Department of Microbiology, Immunology and Parasitology (AR), Louisiana State University Health Sciences Center, New Orleans, Louisiana.
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  • Kyle I. Happel,

    1. From the Pulmonary/Critical Care Medicine(EP, KIH, XST, CMM), Louisiana State University Health Sciences Center, New Orleans, Louisiana; and Department of Microbiology, Immunology and Parasitology (AR), Louisiana State University Health Sciences Center, New Orleans, Louisiana.
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  • Xu S. Teng,

    1. From the Pulmonary/Critical Care Medicine(EP, KIH, XST, CMM), Louisiana State University Health Sciences Center, New Orleans, Louisiana; and Department of Microbiology, Immunology and Parasitology (AR), Louisiana State University Health Sciences Center, New Orleans, Louisiana.
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  • Alistair Ramsay,

    1. From the Pulmonary/Critical Care Medicine(EP, KIH, XST, CMM), Louisiana State University Health Sciences Center, New Orleans, Louisiana; and Department of Microbiology, Immunology and Parasitology (AR), Louisiana State University Health Sciences Center, New Orleans, Louisiana.
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  • Carol M. Mason

    1. From the Pulmonary/Critical Care Medicine(EP, KIH, XST, CMM), Louisiana State University Health Sciences Center, New Orleans, Louisiana; and Department of Microbiology, Immunology and Parasitology (AR), Louisiana State University Health Sciences Center, New Orleans, Louisiana.
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Reprint requests: Carol Mason, MD, Pulmonary/Critical Care Medicine, 1901 Perdido St. no. 3205, New Orleans, LA 70112; Tel.: 504-568-4634; Fax: 504-568-4582; E-mail: mason@lsuhsc.edu

Abstract

Background:  Alcoholics are at heightened risk for developing active tuberculosis. This study evaluates chronic alcohol consumption in a murine model of vaccination with Mycobacterium bovis Bacille Calmette–Guèrin (BCG) and subsequent pulmonary infection with virulent Mycobacterium tuberculosis.

Methods:  BALB/c mice were administered the Lieber–DeCarli liquid ethanol diet or pair-fed the liquid control diet for 3 weeks either before or after subcutaneous vaccination with M. bovis BCG. At least 3 weeks after BCG vaccination, groups of mice on the aforesaid diets were challenged with intratracheal infection with M. tuberculosis H37Rv. Lung mycobacterial burden, and lung and lung-associated lymph node CD4+ lymphocyte production of tuberculosis-specific interferon (IFN)-γ were assayed. Popliteal lymph node lymphocytes from both dietary regimens undergoing BCG vaccination (in the absence of M. tuberculosis infection) were also evaluated for purified protein derivative–induced IFN-γ production by ELISpot assay.

Results:  Mice begun on alcohol prior to vaccination with M. bovis BCG demonstrated impaired control of pulmonary challenge with virulent M. tuberculosis, as well as impaired lung CD4+ and popliteal lymph node T-cell IFN-γ responses. If BCG vaccination was delivered prior to initiation of alcohol feeding, the mice remained protected against a subsequent challenge with M. tuberculosis, and BCG-induced immunity was not impaired in either the lung or the popliteal lymph nodes.

Conclusions:  Alcohol consumption blunts the development of the adaptive immune response to M. bovis BCG vaccination, which impairs the control of a secondary challenge with M. tuberculosis, but only if the alcohol exposure is begun prior to BCG vaccination. These results provide insight into mechanisms by which alcohol consumption impairs antimycobacterial immunity, including in response to vaccination and subsequent pathogenic challenge.

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