Selenium or Selenium Plus Folic Acid–Supplemented Diets Ameliorate Renal Oxidation in Ethanol-Exposed Pups
Article first published online: 6 APR 2012
Copyright © 2012 by the Research Society on Alcoholism
Alcoholism: Clinical and Experimental Research
Volume 36, Issue 11, pages 1863–1872, November 2012
How to Cite
Ojeda, M. L., Nogales, F., Murillo, M. L. and Carreras, O. (2012), Selenium or Selenium Plus Folic Acid–Supplemented Diets Ameliorate Renal Oxidation in Ethanol-Exposed Pups. Alcoholism: Clinical and Experimental Research, 36: 1863–1872. doi: 10.1111/j.1530-0277.2012.01788.x
- Issue published online: 29 OCT 2012
- Article first published online: 6 APR 2012
- Manuscript Accepted: 13 FEB 2012
- Manuscript Received: 19 OCT 2011
- Folic Acid;
- Kidney Oxidation
Ethanol (EtOH) exposure during gestation and lactation induces an oxidative stress in offspring. In kidney, the oxidative damage is the primary pathway to alcohol-induced injury. In this study, we have demonstrated that a diet supplemented with selenium (Se) (0.5 ppm) or with Se (0.5 ppm) + folic acid (8 ppm) administered to EtOH-exposed (20% v/v) dams during gestation and lactation prevents the oxidative EtOH-provoked effects in their offspring's kidneys.
All the studies were performed on 21-day-old pups. Serum, urine, and kidney Se levels were assessed by graphite-furnace atomic absorption spectrometry. Se and creatinine clearance, antioxidant enzyme activities, and lipid and protein peroxidation were determined by a spectrophotometric method in kidney.
Dietary supplementation treatments used could not improve the glomerular filtration function altered by EtOH exposure during gestation and lactation; however, they did improve renal Se deposits, renal development, and renal protein content while decreasing lipid and protein oxidation and modifying antioxidant enzymes' activity.
Se or Se + folic acid supplementations improve renal development and protein content and modify antioxidant enzymes' activity, decreasing lipid and protein oxidation after EtOH exposure. In this context, a double-supplemented diet appears to reduce protein peroxidation more efficiently than the Se-only-supplemented one, probably via superoxide dismutase and catalase.