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Positron Emission Tomography Shows Elevated Cannabinoid CB 1 Receptor Binding in Men with Alcohol Dependence

Authors

  • Alexander Neumeister,

    Corresponding author
    1. Mood and Anxiety Disorders Program, Department of Psychiatry, Mount Sinai School of Medicine, New York, New York
    • Molecular Imaging Program, Department of Psychiatry and Radiology, New York University School of Medicine, New York, New York
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  • Marc D. Normandin,

    1. Department of Diagnostic Radiology, Positron Emission Tomography Center, Yale University School of Medicine, New Haven, Connecticut
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  • James W. Murrough,

    1. Mood and Anxiety Disorders Program, Department of Psychiatry, Mount Sinai School of Medicine, New York, New York
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  • Shannan Henry,

    1. Department of Diagnostic Radiology, Positron Emission Tomography Center, Yale University School of Medicine, New Haven, Connecticut
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  • Christopher R. Bailey,

    1. Mood and Anxiety Disorders Program, Department of Psychiatry, Mount Sinai School of Medicine, New York, New York
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  • David A. Luckenbaugh,

    1. Experimental Therapeutics, Mood and Anxiety Disorders Program, Division of Intramural Research Programs , National Institute of Mental Health, Bethesda, Maryland
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  • Keri Tuit,

    1. Department of Psychiatry, Yale University School of Medicine, New Haven, Connecticut
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  • Ming-Qiang Zheng,

    1. Department of Diagnostic Radiology, Positron Emission Tomography Center, Yale University School of Medicine, New Haven, Connecticut
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  • Isaac R. Galatzer-Levy,

    1. Molecular Imaging Program, Department of Psychiatry and Radiology, New York University School of Medicine, New York, New York
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  • Rajita Sinha,

    1. Department of Psychiatry, Yale University School of Medicine, New Haven, Connecticut
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  • Richard E. Carson,

    1. Department of Diagnostic Radiology, Positron Emission Tomography Center, Yale University School of Medicine, New Haven, Connecticut
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  • Marc N. Potenza,

    1. Department of Psychiatry, Yale University School of Medicine, New Haven, Connecticut
    2. Department of Neurobiology and Child Study Center, Yale University School of Medicine, New Haven, Connecticut
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  • Yiyun Huang

    1. Department of Diagnostic Radiology, Positron Emission Tomography Center, Yale University School of Medicine, New Haven, Connecticut
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  • The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH or NIAAA.

Reprint requests: Alexander Neumeister, MD, Department of Psychiatry and Radiology, New York University School of Medicine, One Park Avenue, 8th floor, room 225, New York, NY 10016; Tel.: 646-754-2232; Fax: 646-754-2300; E-mail: alexander.neumeister@nyumc.org

Abstract

Background

Several lines of evidence link cannabinoid (CB) type 1 (CB 1) receptor-mediated endogenous CB (eCB) signaling to the etiology of alcohol dependence (AD). However, to date, only peripheral measures of eCB function have been collected in living humans with AD and no human in vivo data on the potentially critical role of the brain CB 1 receptor in AD have been published. This is an important gap in the literature, because recent therapeutic developments suggest that these receptors could be targeted for the treatment for AD.

Methods

Medication-free participants were scanned during early abstinence 4 weeks after their last drink. Using positron emission tomography (PET) with a high-resolution research tomograph and the CB 1 receptor selective radiotracer [11 C]OMAR, we determined [11 C]OMAR volume of distribution ( V T) values, a measure of CB 1 receptor density, in a priori selected brain regions in men with AD (= 8, age 37.4 ± 7.9 years; 5 smokers) and healthy control (HC) men (n = 8, age 32.5 ± 6.9 years; all nonsmokers). PET images reconstructed using the MOLAR algorithm with hardware motion correction were rigidly aligned to the subject-specific magnetic resonance (MR) image, which in turn was warped to an MR template. Time–activity curves (TACs) were extracted from the dynamic PET data using a priori selected regions of interest delineated in the MR template space.

Results

In AD relative to HC, [11 C]OMAR V T values were elevated by approximately 20% (= 0.023) in a circuit, including the amygdala, hippocampus, putamen, insula, anterior and posterior cingulate cortices, and orbitofrontal cortex. Age, body mass index, or smoking status did not influence the outcome.

Conclusions

These findings agree with preclinical evidence and provide the first, albeit still preliminary in vivo evidence suggesting a role for brain CB 1 receptors in AD. The current study design does not answer the important question of whether elevated CB 1 receptors are a preexisting vulnerability factor for AD or whether elevations develop as a consequence of AD.

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