Evidence for Possible Period 2 Gene Mediation of the Effects of Alcohol Exposure During the Postnatal Period on Genes Associated with Maintaining Metabolic Signaling in the Mouse Hypothalamus
Article first published online: 23 JUL 2012
Copyright © 2012 by the Research Society on Alcoholism
Alcoholism: Clinical and Experimental Research
Volume 37, Issue 2, pages 263–269, February 2013
How to Cite
Agapito, M. A., Barreira, J. C., Logan, R. W. and Sarkar, D. K. (2013), Evidence for Possible Period 2 Gene Mediation of the Effects of Alcohol Exposure During the Postnatal Period on Genes Associated with Maintaining Metabolic Signaling in the Mouse Hypothalamus. Alcoholism: Clinical and Experimental Research, 37: 263–269. doi: 10.1111/j.1530-0277.2012.01871.x
- Issue published online: 1 FEB 2013
- Article first published online: 23 JUL 2012
- Manuscript Accepted: 4 MAY 2012
- Manuscript Received: 14 FEB 2012
- National Institute of Health. Grant Numbers: R01 AA015718, R37 AA08757
- Circadian Rhythm;
- Fetal Alcohol Spectrum Disorders;
- Metabolic Disorders;
- POMC Neurons
Animals exposed to alcohol during the developmental period develop circadian disturbances and metabolic problems that often persist during their adult period. In order to study whether alcohol and the circadian clock interact to alter metabolic signaling in the hypothalamus, we determined whether postnatal alcohol feeding in mice permanently alters metabolic sensing in the hypothalamus. Furthermore, we evaluated whether the effect of circadian disruption via Period 2 (Per2) gene mutation prevents alcohol's effects on metabolic signaling in the hypothalamus.
Per2 mutant and wild-type male and female mice of the same genetic background were given a milk formula containing ethanol (EtOH; 11.34% vol/vol) from postnatal day (PD) 2 to 7 and used for gene expression and peptide level determinations in the hypothalamus at PD7 and PD90.
We report here that postnatal alcohol feeding reduces the expression of proopiomelanocortin (Pomc) gene and production of β-endorphin and α-melanocyte stimulating hormone (α-MSH) in the hypothalamus that persists into adulthood. In addition, expressions of metabolic sensing genes in the hypothalamus were also reduced as a consequence of postnatal alcohol exposure. These effects were not sex-specific and were observed in both males and females. Mice carrying a mutation of the Per2 gene did not show any reductions in hypothalamic levels of Pomc and metabolic genes and β-endorphin and α-MSH peptides following alcohol exposure.
These data suggest that early-life exposure to alcohol alters metabolic sensing to the hypothalamus possibly via regulating Per2 gene and/or the cellular circadian clock mechanism.