1Drs. De Fanis and Wang contributed equally to this work.
T-Lymphocytes Expressing CC Chemokine Receptor-5 Are Increased in Frail Older Adults
Article first published online: 1 APR 2008
© 2008, Copyright the Authors. Journal compilation © 2008, The American Geriatrics Society
Journal of the American Geriatrics Society
Volume 56, Issue 5, pages 904–908, May 2008
How to Cite
De Fanis, U., Wang, G. C., Fedarko, N. S., Walston, J. D., Casolaro, V. and Leng, S. X. (2008), T-Lymphocytes Expressing CC Chemokine Receptor-5 Are Increased in Frail Older Adults. Journal of the American Geriatrics Society, 56: 904–908. doi: 10.1111/j.1532-5415.2008.01673.x
- Issue published online: 1 APR 2008
- Article first published online: 1 APR 2008
- CCR5+ T-lymphocytes
OBJECTIVES: To evaluate the frequencies of T-lymphocytes expressing CC chemokine receptor-5 (CCR5+ T-cells) and their relationship with frailty in older adults.
DESIGN: Case-control study with an age-, race-, and sex-matched design.
SETTING: General Clinical Research Center.
PARTICIPANTS: Community-dwelling adults aged 72 and older from Baltimore, Maryland.
METHODS: Frailty was determined using five validated criteria: weakness, slow walking speed, fatigue, low physical activity, and weight loss. Those meeting three or more of these five criteria were defined as frail and those with none as nonfrail. Complete blood counts were performed to obtain peripheral lymphocyte counts using an automated (Coulter) counter. Peripheral blood was collected for surface immunofluorescent staining of CCR5 and other T-cell markers.
RESULTS: Twenty-six frail and matched nonfrail participants (mean age±standard deviation 83.8±5.3, range 72–94) completed the study. Frail participants had higher CCR5+, CCR5+CD8+, and CCR5+CD45RO− T-cell counts than matched nonfrail controls (349±160/mm3 vs 194±168/mm3, P=.02; 208±98/mm3 vs 105±62/mm3, P=.02; and 189±149/mm3 vs 52±36/mm3, P=.01; respectively). Furthermore, there was a trend toward graded increase in these T-cell counts across the frailty scores in frail participants (e.g., CCR5+CD8+ counts of 123±52/mm3, 248±115/mm3, and 360±215/mm3 for those with frailty scores of 3, 4, and 5, respectively).
CONCLUSION: These initial results suggest an expansion of the CCR5+ T-cell subpopulation in frailty. They provide a basis for further characterization of CCR5+ T-cells and their role in frailty, with potential therapeutic implications.