Presented at the 13th Annual Scientific Meeting of the European College of Veterinary Surgeons, Prague, Czech Republic, July 2–4, 2004.
Induction of a Barrier Membrane to Facilitate Reconstruction of Massive Segmental Diaphyseal Bone Defects: An Ovine Model
Version of Record online: 7 JUL 2006
Volume 35, Issue 5, pages 445–452, July 2006
How to Cite
VIATEAU, V., GUILLEMIN, G., CALANDO, Y., LOGEART, D., OUDINA, K., SEDEL, L., HANNOUCHE, D., BOUSSON, V. and PETITE, H. (2006), Induction of a Barrier Membrane to Facilitate Reconstruction of Massive Segmental Diaphyseal Bone Defects: An Ovine Model. Veterinary Surgery, 35: 445–452. doi: 10.1111/j.1532-950X.2006.00173.x
- Issue online: 7 JUL 2006
- Version of Record online: 7 JUL 2006
- Submitted September 2005; Accepted March 2006
Objectives— To report an ovine model that can be used to evaluate the efficacy of bone substitutes for repair of segmental diaphyseal bone defects.
Study Design— Experimental study.
Animals— Eleven 2-year-old Pré-Alpes Sheep.
Methods— Mid-diaphyseal metatarsal bone defects (25 mm long) were stabilized by a dynamic compression plate over a polymethylmethacrylate (PMMA) cement spacer, and by external coaptation. The PMMA spacer was removed at 6 weeks by incising the encapsulating membrane. The defect remained unfilled (Group 1; n=5) or was filled with morselized autologous corticocancellous graft (Group 2; n=6), the membrane sutured closed, and external coaptation applied for 6 months, when healing was evaluated.
Results— Radiographic, computed tomographic, and histologic examinations at 6 months after the 2nd surgery revealed non-union in ungrafted defects whereas grafted defects showed bone healing. The induced membrane had blood vessels, CBFA1+ cells, and very few macrophages entrapped in a collagenous tissue positive for type I collagen.
Conclusion— This ovine metatarsal defect model resulted in a critical-size defect (non-union) that healed when grafted. The PMMA-induced membrane constrained the graft, was well vascularized, and may have osteogenic properties.
Clinical Relevance— This model may be useful to evaluate new strategies in bone tissue engineering because the PMMA-induced membrane may help confine bone morphogenetic proteins, skeletal stem cells, or other agents to the defect cavity where they could be useful to enhance bone formation.