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Recombinant Human Bone Morphogenetic Protein-2 in Absorbable Collagen Sponge Enhances Bone Healing of Tibial Osteotomies in Dogs

Authors

  • MARIA L. E. FARIA DVM, PhD,

    1. Comparative Orthopaedic Research Laboratory, Departments of Medical Sciences and Surgical Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI and Fort Dodge Animal Health, Princeton, NJ.
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  • YAN LU MD,

    1. Comparative Orthopaedic Research Laboratory, Departments of Medical Sciences and Surgical Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI and Fort Dodge Animal Health, Princeton, NJ.
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  • KATHLEEN HEANEY DVM,

    1. Comparative Orthopaedic Research Laboratory, Departments of Medical Sciences and Surgical Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI and Fort Dodge Animal Health, Princeton, NJ.
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  • RAJESH K. UTHAMANTHIL MVSc, PhD,

    1. Comparative Orthopaedic Research Laboratory, Departments of Medical Sciences and Surgical Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI and Fort Dodge Animal Health, Princeton, NJ.
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  • PETER MUIR BVSc, MVetClinStud, PhD, Diplomate ACVS,

    1. Comparative Orthopaedic Research Laboratory, Departments of Medical Sciences and Surgical Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI and Fort Dodge Animal Health, Princeton, NJ.
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  • MARK D. MARKEL DVM, PhD, Diplomate ACVS

    1. Comparative Orthopaedic Research Laboratory, Departments of Medical Sciences and Surgical Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI and Fort Dodge Animal Health, Princeton, NJ.
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  • Presented in part at the 2004 ACVS Veterinary Symposium, Denver, CO, October 2004.

Address reprint requests to Mark D. Markel, DVM, PhD, Department of Medical Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI 53706. E-mail: markelm@svm.vetmed.wisc.edu.

Abstract

Objective— To compare the efficacy of 2 doses of recombinant human bone morphogenetic protein-2 (rhBMP-2) on tibial osteotomy healing in dogs.

Study Design— Experimental, randomized complete block (n=7).

Animals— Adult female dogs (n=21).

Methods— Right midshaft tibial osteotomies were created and stabilized with a 1-mm gap using type I external fixators. Seven dogs were untreated controls and 14 with osteotomies were treated with either 0.05 or 0.2 mg/mL rhBMP-2 delivered in an absorbable collagen sponge (ACS). At 8 weeks, dogs were euthanatized and bones were mechanically tested and examined by microscopy.

Results— Bone healing based on radiographic scoring, was significantly improved in dogs treated with 0.2 mg/mL of rhBMP-2 compared with the other groups; these tibiae were also significantly stronger and stiffer than 0.05 mg/mL rhBMP-2 and control osteotomized tibiae. Histologic scores were significantly better for 0.2 mg/mL rhBMP-2 group than 0.05 mg/mL rhBMP-2 group, but neither was significantly different from control.

Conclusions— rhBMP-2 in ACS at a concentration of 0.2 mg/mL improves healing of tibial osteotomies in dogs compared with untreated controls and 0.05 mg/mL rhBMP-2 based on force plate analysis and radiographic evaluation. This was not confirmed histologically but treated bones had improved mechanical properties at 8 weeks.

Clinical Relevance— After a long bone fracture, dogs may face a long recovery period before full return of limb function. rhBMP-2, in association with good fracture fixation principles, may enhance bone healing in dogs with diaphyseal fractures.

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