Presented at the American College of Veterinary Surgeons Annual Symposium, Chicago, IL, October 2007.
Validation of a Rapid Parathyroid Hormone Assay and Intraoperative Measurement of Parathyroid Hormone in Dogs with Benign Naturally Occurring Primary Hyperparathyroidism
Article first published online: 30 DEC 2008
© Copyright 2009 by The American College of Veterinary Surgeons
Volume 38, Issue 1, pages 122–132, January 2009
How to Cite
HAM, K., GREENFIELD, C. L., BARGER, A., SCHAEFFER, D., EHRHART, E. J., PINKERTON, M. and VALLI, V. E. O. (2009), Validation of a Rapid Parathyroid Hormone Assay and Intraoperative Measurement of Parathyroid Hormone in Dogs with Benign Naturally Occurring Primary Hyperparathyroidism . Veterinary Surgery, 38: 122–132. doi: 10.1111/j.1532-950X.2008.00457.x
Work was completed at the Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois, Urbana, IL.
Supported by a grant provided by the University of Illinois Companion Animal Memorial Fund and the Northern Illinois Veterinary Medical Association Graduate Research Award.
- Issue published online: 30 DEC 2008
- Article first published online: 30 DEC 2008
- Submitted March 2008; Accepted July 2008
Objectives— To (1) validate a rapid chemiluminescent parathyroid hormone (PTH) assay, (2) determine it's usefulness locating a parathyroid nodule(s), and (3) determine if >50% decrease in PTH corresponds with excision of autonomously functioning parathyroid tissue.
Study Design— Prospective cohort study.
Animals— Dogs (n=12) with naturally occurring primary hyperparathyroidism and 25 healthy dogs.
Methods— The assay was validated with linearity, precision, and intermethod comparison. Preoperative and postoperative systemic plasma PTH concentrations, measured from saphenous venous blood, were compared. Intraoperative local PTH concentrations were measured in right and left jugular venous blood before and after surgical excision of the grossly abnormal parathyroid gland(s).
Results— Within run and day-to-day precisions were acceptable (coefficient of variation <15%). Dilutional parallelism was used to demonstrate high correlation between measured and calculated PTH concentrations (R2=0.99). The assay methods had good correlation but numerical results of the rapid assay were usually lower than the immunoradiometric assay. Seven of 12 dogs had uniglandular disease and five had multiglandular disease. Systemic and local PTH concentrations decreased >50% in all the dogs after excision of the parathyroid gland(s). Mean preoperative systemic plasma PTH concentrations were significantly higher than mean postoperative systemic concentrations. Local PTH concentrations could not be used reliably to differentiate the side of the autonomously functioning gland(s). Hypercalcemia resolved postoperatively in all the dogs.
Conclusion— This assay measures PTH in dogs. Rapid PTH measurement provided documentation of decreased PTH concentration after removal of autonomously functioning parathyroid tissue.
Clinical Relevance— Use of this assay allows documentation of a significant decrease in PTH concentration after excision of autonomously functioning parathyroid tissue.