Dr. Lafuente's present address is the Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Washington State University, Pullman, WA 99164. Study performed at the College of Veterinary Medicine, Autonomous University of Barcelona, the College of Odontology, University of Barcelona, and College of Physics, University of Barcelona, Barcelona, Spain. Financial support provided by a grant from the Generalitat de Catalunya. Presented in part at the 2nd World Veterinary Orthopedic Congress and 33rd Annual VOS Meeting, Keystone, CO, February 25–March 4, 2006.
Experimental Study of Bone Lengthening in Dogs by Means of Backscattered Scanning Electron Microscopy
Article first published online: 1 APR 2009
© Copyright 2009 by The American College of Veterinary Surgeons
Volume 38, Issue 3, pages 388–397, April 2009
How to Cite
LAFUENTE, P., FRANCH, J., DURALL, I. and MANZANARES, C. (2009), Experimental Study of Bone Lengthening in Dogs by Means of Backscattered Scanning Electron Microscopy. Veterinary Surgery, 38: 388–397. doi: 10.1111/j.1532-950X.2009.00505.x
- Issue published online: 1 APR 2009
- Article first published online: 1 APR 2009
- Submitted October 2007; Accepted April 2008
Objective— To describe the morphology of calcified tissues involved in distraction osteogenesis (DO) by means of backscattered scanning electron microscopy (BS-SEM).
Study Design— Experimental study.
Animals— Adult female Beagle dogs (n=12).
Methods— Nonsimultaneous and bilateral transverse mid-diaphyseal osteotomies performed in tibiae were stabilized and distracted by a Type Ia external skeletal fixation device. After a latency period of 5 days, distraction was applied at a rate of 0.5 mm every 12 hours for 10 days. Then, the external fixator was maintained in a static mode during the consolidation period until bone healing or euthanasia at 1, 2, 3, 4, 6, 8, 10, 12, 14, and 18 weeks after operations, whichever came first. Distracted regions were isolated and their structure was examined by BS-SEM.
Results— Calcified chondroid tissue was prominent during distraction and calcified cartilaginous tissue during consolidation; both tissues were successively replaced by woven, lamellar, and osteonal bone.
Conclusions— In osteotomized tibia, chondroid tissue is the main component of the mineralization front during distraction, calcified cartilaginous tissue during consolidation, and then both tissues are replaced by woven, lamellar, and osteonal bone. The ossification mechanism of distraction callus is transchondroidal.
Clinical Relevance— BS-SEM is an effective technique for studying progression of bone healing during DO. The presence of chondroid tissue during DO explains why callus mineralization occurs more rapidly during distraction than during static stabilization.