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Evaluation of a Collagenase Generated Osteoarthritis Biomarker in the Synovial Fluid from Elbow Joints of Dogs with Medial Coronoid Disease and Unaffected Dogs

Authors

  • ADAM PRINK DVM,

    1. JD Wheat Veterinary Orthopedic Research Laboratory, the Department of Surgical and Radiological Sciences, School of Veterinary Medicine, University of California–Davis, Davis, CA
    2. William R. Pritchard Veterinary Medical Teaching Hospital, University of California–Davis, Davis, CA
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  • KEI HAYASHI DVM, PhD, Diplomate ACVS,

    1. JD Wheat Veterinary Orthopedic Research Laboratory, the Department of Surgical and Radiological Sciences, School of Veterinary Medicine, University of California–Davis, Davis, CA
    2. William R. Pritchard Veterinary Medical Teaching Hospital, University of California–Davis, Davis, CA
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  • SUN-YOUNG KIM DVM, MS,

    1. JD Wheat Veterinary Orthopedic Research Laboratory, the Department of Surgical and Radiological Sciences, School of Veterinary Medicine, University of California–Davis, Davis, CA
    2. William R. Pritchard Veterinary Medical Teaching Hospital, University of California–Davis, Davis, CA
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  • JAMES KIM DVM,

    1. JD Wheat Veterinary Orthopedic Research Laboratory, the Department of Surgical and Radiological Sciences, School of Veterinary Medicine, University of California–Davis, Davis, CA
    2. William R. Pritchard Veterinary Medical Teaching Hospital, University of California–Davis, Davis, CA
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  • AMY KAPATKIN DVM, MS, Diplomate ACVS

    1. JD Wheat Veterinary Orthopedic Research Laboratory, the Department of Surgical and Radiological Sciences, School of Veterinary Medicine, University of California–Davis, Davis, CA
    2. William R. Pritchard Veterinary Medical Teaching Hospital, University of California–Davis, Davis, CA
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Corresponding author: Dr. Kei Hayashi, DVM, PhD, Diplomate ACVS, Department of Surgical and Radiological Sciences, School of Veterinary Medicine, University of California–Davis, Davis, CA 95616 8745. E-mail: khayashi@ucdavis.edu.

Abstract

Objective— To evaluate whether synovial fluid concentrations of an osteoarthritis biomarker in dysplastic canine elbows with medial coronoid disease (MCD) are elevated compared with unaffected elbows and to determine if these concentrations correlate to the degree of articular cartilage damage.

Study Design— Cross sectional clinical study.

Animals— Dogs (n=19; 35 elbows) with MCD and dogs (8; 16 elbows) with unaffected elbows.

Methods— Concentrations of a collagenase-generated cleavage neoepitope of type II collagen (Col2-3/4Clong mono, or C2C) in joint fluid from elbows were analyzed and compared between dogs with MCD and unaffected dogs. Correlation of C2C concentration with subjective grading of articular cartilage surface damage was also evaluated.

Results— Mean (±SD) C2C concentration from MCD dogs was significantly higher (112.3±24.8 ng/mL) than in unaffected dogs (76.1±16.9 ng/mL; P<.05). There was a moderate correlation between cartilage damage grade and increasing C2C concentrations (P<.05, r=0.62)

Conclusion— C2C concentrations are elevated in the synovial fluid of dogs with MCD compared with unaffected elbows, and a moderate, significant correlation was identified between these concentrations and subjective grading of articular cartilage damage.

Clinical Relevance— This preliminary data suggest that C2C concentrations in synovial fluid may have potential as a biomarker for diagnosis of articular cartilage damage associated with MCD and as a means of objectively determining the degree of articular cartilage damage.

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