Presented in part at the Resident Forum, 15th Annual Scientific Meeting of the European College of Veterinary Surgeons, Seville, Spain, June 29–July 1, 2006.
Increased Cartilage Oligomeric Matrix Protein Concentrations in Equine Digital Flexor Tendon Sheath Synovial Fluid Predicts Intrathecal Tendon Damage
Article first published online: 15 NOV 2010
© Copyright 2010 by The American College of Veterinary Surgeons
Volume 40, Issue 1, pages 54–58, January 2011
How to Cite
Smith, M. R. W., Wright, I. M., Minshall, G. J., Dudhia, J., Verheyen, K., Heinegård, D. and Smith, R. K. W. (2011), Increased Cartilage Oligomeric Matrix Protein Concentrations in Equine Digital Flexor Tendon Sheath Synovial Fluid Predicts Intrathecal Tendon Damage. Veterinary Surgery, 40: 54–58. doi: 10.1111/j.1532-950X.2010.00751.x
- Issue published online: 3 JAN 2011
- Article first published online: 15 NOV 2010
- Submitted March 2009Accepted October 2009
Objectives: To evaluate digital flexor tendon sheath (DFTS) synovial fluid cartilage oligomeric matrix protein (COMP) concentrations as a molecular marker for intrathecal pathology.
Study Design: Case control study.
Animals: Horses (n=46) with DFTS tenosynovitis; 23 fresh cadaver horses.
Methods: DFTS synovial fluid samples were collected from clinical cases with noninfected DFTS tenosynovitis and from control DFTS. Clinical and surgical findings were recorded, and dissection of control limbs was performed to confirm the DFTS to be grossly normal. Synovial fluid COMP was quantified using a homologous competitive inhibition ELISA.
Results: Abnormalities were identified tenoscopically: intrathecal tendon/ligament tearing was identified in 37 cases and 9 had other lesions. In control horses, synovial fluid COMP was higher in younger horses. Clinical cases with intrathecal tendon/ligament tearing had higher synovial fluid COMP than either clinical cases with other lesions, or controls. In horses ≥5 years old, the sensitivity and specificity of the assay was high for diagnosing intrathecal tendon/ligament tearing.
Conclusions: COMP concentrations in DFTS synovial fluid were significantly greater than those in normal horses with noninfected tenosynovitis caused by intrathecal tendon/ligament tearing, but not by other lesions.