Effects of Aerobic and Anaerobic Fluid Collection on Biochemical Analysis of Peritoneal Fluid in Healthy Horses and Horses with Colic
Article first published online: 23 DEC 2010
© Copyright 2010 by The American College of Veterinary Surgeons
Volume 40, Issue 1, pages 40–45, January 2011
How to Cite
Romero, A. E., Nieto, J. E., Dechant, J. E., Hopper, K. and Aleman, M. (2011), Effects of Aerobic and Anaerobic Fluid Collection on Biochemical Analysis of Peritoneal Fluid in Healthy Horses and Horses with Colic. Veterinary Surgery, 40: 40–45. doi: 10.1111/j.1532-950X.2010.00767.x
- Issue published online: 3 JAN 2011
- Article first published online: 23 DEC 2010
- Submitted December 2008Accepted January 2010
Objective: To determine whether in healthy horses and those with colic, exposure of peritoneal fluid to room air affects values obtained on biochemical analysis.
Study Design: Prospective study.
Animals: Adult horses with a primary complaint of acute abdominal pain (n=29) and 12 healthy horses.
Methods: Peritoneal fluid was aseptically collected under aerobic and anaerobic conditions. After collection, pH, PCO2, PO2, HCO3−, Na+, ionized Ca2+, K+, lactate, and glucose were immediately measured using a commercial blood gas analyzer. Biochemical variables were compared between aerobically and anaerobically obtained samples using a paired t-test.
Results: In healthy horses, peritoneal fluid samples collected under anaerobic conditions had higher PCO2 and ionized Ca2+ and lower PO2, HCO3−, and pH compared with samples exposed to air. No differences were observed for K+, Na+, glucose, and lactate. In horses with colic, samples collected anaerobically had higher PCO2, ionized Ca2+, Na+, and glucose and lower PO2, HCO3−, and pH value compared with samples exposed to air. No differences were observed for K+ and lactate.
Conclusion: Exposure of peritoneal fluid to room air had a significant effect on pH, PCO2, PO2, and variables associated or dependent on changes in pH such as HCO3− and ionized Ca2+. Interpretation of biochemical analysis of peritoneal fluid may be influenced by sample collection method.