Original Article - Research
Comparison of pH, Lactate, and Glucose Analysis of Equine Synovial Fluid using a Portable Clinical Analyzer with a Bench-Top Blood Gas Analyzer
Article first published online: 19 JUL 2011
© Copyright 2011 by The American College of Veterinary Surgeons
Volume 40, Issue 7, pages 811–816, October 2011
How to Cite
Dechant, J. E., Symm, W. A. and Nieto, J. E. (2011), Comparison of pH, Lactate, and Glucose Analysis of Equine Synovial Fluid using a Portable Clinical Analyzer with a Bench-Top Blood Gas Analyzer. Veterinary Surgery, 40: 811–816. doi: 10.1111/j.1532-950X.2011.00854.x
- Issue published online: 5 OCT 2011
- Article first published online: 19 JUL 2011
- Manuscript Accepted: OCT 2010
- Manuscript Received: JAN 2010
To compare agreement between a portable clinical analyzer and laboratory-based bench-top analyzer for analysis of pH, lactate, and glucose concentrations in synovial fluid.
Prospective experimental study.
Clinically normal horses (n=8); 6 horses euthanatized for reasons unrelated to the study; 11 horses that had synoviocentesis for reasons other than sepsis; 7 horses that had synoviocentesis for evaluation of sepsis; and 2 horses without recorded clinical data. Median age of horses was 8 years (range, 1 day to 24 years).
Supernatant from each synovial fluid sample was analyzed for pH, lactate, and glucose concentrations using an ABL 705 laboratory-based bench-top analyzer and i-STAT portable clinical analyzer. Bland–Altman plots were constructed and concordance analysis performed to determine bias and agreement between the 2 analyzers.
There was acceptable agreement between analyzers for lactate and glucose concentrations, with biases of 0.198 mmol/L and 9 mg/dL and concordance correlation coefficients of 0.97 and 0.96 for lactate and glucose, respectively. The agreement between analyzers for pH was not acceptable, with a bias of −0.057 and concordance correlation coefficient of 0.89.
This study found that the portable clinical analyzer performed similarly to the bench-top blood gas analyzer for evaluation of lactate and glucose concentrations, but not pH, in synovial fluid.