Original Article - Research
Activation of Equine Platelet-Rich Plasma: Comparison of Methods and Characterization of Equine Autologous Thrombin
Article first published online: 28 JUN 2012
© Copyright 2012 by The American College of Veterinary Surgeons
Volume 41, Issue 7, pages 784–794, October 2012
How to Cite
Textor, J. A. and Tablin, F. (2012), Activation of Equine Platelet-Rich Plasma: Comparison of Methods and Characterization of Equine Autologous Thrombin. Veterinary Surgery, 41: 784–794. doi: 10.1111/j.1532-950X.2012.01016.x
- Issue published online: 17 OCT 2012
- Article first published online: 28 JUN 2012
- Manuscript Accepted: MAR 2012
- Manuscript Received: OCT 2011
To investigate and compare clinically relevant Platelet-rich plasma (PRP) activation methods.
PRP was prepared from 6 equine subjects. Activation of the PRP was performed by 4 methods (autologous thrombin, bovine thrombin, calcium chloride (CaCl2), or freeze-thaw). The resultant PDGF-BB (where PDGF is platelet-derived growth factor) and TGFβ1 (where TGFβ is transforming growth factor beta) levels in PRP releasates were quantified by Enzyme-linked immunosorbent assay (ELISA) and compared. Growth factor contents were also compared between platelet-rich clots produced by thrombin or CaCl2. The composition and function of equine autologous thrombin were characterized by Western blot analysis and platelet aggregometry.
CaCl2 (23 mM) activation of PRP yielded significantly greater PDGF release than did any other method. TGFβ release was comparable after PRP activation by CaCl2, bovine thrombin, and freeze thaw. Autologous thrombin was significantly less effective than all other activation methods in eliciting platelet growth factor release and induced significantly less platelet aggregation than bovine thrombin at 5 U/mL. Clots retained substantial concentrations of growth factor, and the amount in the releasate versus the clot differed between activation methods.
PRP activation methods differ in terms of growth factor output as well as logistical considerations. Autologous thrombin is not recommended for PRP activation. CaCl2 (23 mM) is an effective and inexpensive method of PRP activation. The PRP releasate derived from CaCl2 activation contains 80% of the total PDGF content and is easily produced, making it a convenient product for clinical use.