Treatment of Pythiosis in Equine Limbs Using Intravenous Regional Perfusion of Amphotericin B

Authors

  • Renata G. S. Dória DVM, MS, PhD,

    Corresponding author
    • Department of Animal Science, Faculty of Animal Science, and Food Engineering, University of São Paulo, São Paulo, Brazil
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  • Silvio H. Freitas DVM, MS, PhD,

    1. Department of Veterinary Medicine and Surgery, School of Veterinary Medicine, University of Cuiabá, Mato Grosso, Brazil
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  • Renata L. Linardi DVM, MS, PhD,

    1. Equine Health Studies Program, Department of Veterinary Clinical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA
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  • Fábio de Souza Mendonça DVM, MS, PhD,

    1. Department of Morphology and Physiology, Federal Rural University of Pernambuco, Pernambuco, Brazil
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  • Laura P. Arruda DVM, MS,

    1. Department of Veterinary Pathology, School of Veterinary Medicine, University of Cuiabá, Mato Grosso, Brazil
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  • Fabiana M. Boabaid DVM,

    1. Department of Veterinary Pathology, School of Veterinary Medicine, Rio Grande do Sul Federal University, Rio Grande do Sul, Brazil
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  • Carlos A. A. Valadão DVM, MS, PhD

    1. Department of Veterinary Medicine and Surgery, School of Agrarian Sciences and Veterinary Medicine, São Paulo State University, São Paulo, Brazil
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  • Contract grant sponsor: University of Cuiaba; Contract grant sponsor: Cristalia Chemical Products and Pharmaceutics.

Corresponding Author

Renata G. S. Dória, DVM MS PhD, Rua Ivo Bellodi, 21, Jardim Eldorado, Jaboticabal, SP 14.887-106, Brazil

E-mail: redoria@usp.br

Abstract

Objective

To evaluate the effects of intravenous regional limb perfusion (IRLP) administration of amphotericin B in horses to treat pythiosis after surgical excision and thermocautery.

Study Design

Case series.

Animals

Horses (n = 12) with Pythium insidiosum infection of the distal aspect of the thoracic or pelvic limbs.

Methods

After surgical excision of granulation tissue and thermocautery, 50 mg amphotericin B was administered by IRLP through a catheter placed in a superficial vein of the affected limb next to the lesion after placing a tourniquet above the injection site. The lesions and locomotor system were evaluated before treatment and at 7, 14, 21, 28, 35, and 60 days.

Results

Ninety-two percent of horses treated with amphotericin B had complete lesion resolution 35 or 60 days after 1 or 2 IRLP treatments, respectively. IRLP induced limb edema and pain during regional palpation in 42%, and inflammation of the injection site in 33% of horses; however these signs resolved after 14 days.

Conclusions

IRLP administration of amphotericin B was effective for treating pythiosis in equine limbs, resolving infection with manageable side effects.

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