Original Article: Research
Qualitative and quantitative evaluation of avian demineralized bone matrix in heterotopic beds
Version of Record online: 1 OCT 2013
© Copyright 2013 by The American College of Veterinary Surgeons
Volume 42, Issue 8, pages 963–970, November 2013
How to Cite
Reza Sanaei, M., Abu, J., Nazari, M., A.B., M. Z. and Allaudin, Z. N. (2013), Qualitative and quantitative evaluation of avian demineralized bone matrix in heterotopic beds. Veterinary Surgery, 42: 963–970. doi: 10.1111/j.1532-950X.2013.12057.x
- Issue online: 28 OCT 2013
- Version of Record online: 1 OCT 2013
- Manuscript Accepted: 30 MAR 2012
- Manuscript Received: 22 FEB 2011
- Research University Grant Scheme (RUGS). Grant Number: 04-03-08-0459RU
- Universiti Putra Malaysia
To evaluate the osteogenic potential of avian demineralized bone matrix (DBM) in the context of implant geometry.
Rock pigeons (n = 24).
Tubular and chipped forms of DBM were prepared by acid demineralization of long bones from healthy allogeneic donors and implanted bilaterally into the pectoral region of 24 pigeons. After euthanasia at 1, 4, 6, 8, 10, and 12 weeks, explants were evaluated histologically and compared by means of quantitative (bone area) and semi quantitative measures (scores).
All explants had new bone at retrieval with the exception of tubular implants at the end of week 1. The most reactive part in both implants was the interior region between the periosteal and endosteal surfaces followed by the area at the implant-muscle interface. Quantitative measurements demonstrated a significantly (P = .012) greater percentage of new bone formation induced by tubular implants (80.28 ± 8.94) compared with chip implants (57.64 ± 3.12). There was minimal inflammation.
Avian DBM initiates heterotopic bone formation in allogeneic recipients with low grades of immunogenicity. Implant geometry affects this phenomenon as osteoconduction appeared to augment the magnitude of the effects in larger tubular implants.