In vivo PLT increments after transfusions of WBC-reduced PLT concentrates stored for up to 7 days
Article first published online: 11 MAR 2004
Volume 44, Issue 3, pages 330–336, March 2004
How to Cite
Dijkstra-Tiekstra, M.J., Pietersz, R.N.I., Hendriks, E.C.M., Reesink, H.W. and Huijgens, P.C. (2004), In vivo PLT increments after transfusions of WBC-reduced PLT concentrates stored for up to 7 days. Transfusion, 44: 330–336. doi: 10.1111/j.1537-2995.2003.00622.x
- Issue published online: 11 MAR 2004
- Article first published online: 11 MAR 2004
- Received for publication April 29, 2003; revision received July 3, 2003, and accepted August 27, 2003.
BACKGROUND: Bacterial screening and improvement of storage conditions of leukoreduced PLT concentrates (LR-PCs) allows extension of their storage period from 5 to 7 days.
STUDY DESIGN AND METHODS: For in vitro studies, 40 LR-PCs made from five buffy coats and plasma were studied for 8 days. For in vivo studies, routinely produced LR-PCs stored for 2 to 7 days after blood collection were administered to clinically stable thrombocytopenic patients. CI1 h was calculated after 353 transfusions (67 patients), and CCI1 h, after 195 transfu-sions (55 patients), with pretransfusion PLT counts of not greater than 20 × 109 per mL.
RESULTS: Storage experiments showed that the pH of LR-PCs remained greater than 6.8 for 8 days, provided that the PLT concentration was less than 1.3 × 109 per mL. Routinely produced LR-PCs had a volume of 282 ± 15 mL (n = 10,193) and contained 329 × 109± 40 × 109 PLTs (n = 3467). For 7-day-old LR-PCs, 76 of 78 (97%) of the transfusions resulted in a CI1 h of at least 10 and 37 of 39 (95%) in a CCI1 h of at least 7.5, which indicated levels for successfulness. Mean ± SE values of CI1 h and CCI1 h of 7-day-old LR-PCs were 28.7 ± 2.3 (n = 78) and 19.0 ± 2.0 (n = 39), respectively. No significant differences were observed between 5- and 7-day-old LR-PCs transfused with respect to CI1 h and CCI1 h values.
CONCLUSION: In vitro and in vivo studies showed that LR-PCs can be stored for up to 7 days with excellent clinical results, provided that they are routinely screened for bacterial contamination.