From the American Red Cross-North Central Blood Services, St. Paul, Minnesota; the Immunohematology Laboratory, New York Blood Center, New York, New York; Skåne Blood Center, University Hospital, Lund, Sweden; and the Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland.
Evidence that Hy– RBCs express weak Joa antigen
Version of Record online: 17 FEB 2004
Volume 44, Issue 2, pages 170–172, February 2004
How to Cite
Scofield, T. L., Miller, J. P., Storry, J. R., Rios, M. and Reid, M. E. (2004), Evidence that Hy– RBCs express weak Joa antigen. Transfusion, 44: 170–172. doi: 10.1111/j.1537-2995.2004.00627.x
Funded in part by an NIH Specialized Center of Research (SCOR) grant in transfusion medicine and biology (HL54459).
- Issue online: 17 FEB 2004
- Version of Record online: 17 FEB 2004
- Received for publication July 8, 2003; revision received September 10, 2003, and accepted September 11, 2003.
BACKGROUND: RBCs of the Hy– phenotype have, in the past, been typed as Gy(a+w), Hy–, Jo(a–), and RBCs with the Jo(a–) phenotype type Gy(a+), Hy+w, and Jo(a–). Anti-Hy and anti-Joa are difficult to identify mainly because appropriate reagent RBCs are poorly characterized. Historically, anti-Joa has not reacted with RBCs with either phenotype. This report describes a case of an anti-Joa that shows Hy– RBCs express some Joa antigen, albeit weakly.
CASE REPORT: Anti-Joa was identified in a serum sample of a 71-year-old woman. The antibody reacted 1+ to 2+ by the IAT with all untreated and ficin-treated panel RBCs and did not react with Gy(a–) RBCs and Jo(a–) RBCs. Unexpectedly, the serum sample reacted weakly with six of eight RBC samples with the Hy– phenotype. The anti-Joa was adsorbed onto and eluted from Hy– RBCs, indicating the presence of weak Joa antigen. The patient's RBCs typed Gy(a+), Hy+, Jo(a–). DNA studies using PCR-RFLP analysis showed the patient to be homozygous for the JO allele, which is consistent with the serologically determined Jo(a–) status.
CONCLUSION: The DNA and serologic evidence of this case show that Hy– RBCs may express low levels of Joa antigen, which contradicts previously published data concerning the Joa type of Hy– RBCs.