Release of potential immunomodulatory factors during platelet storage

Authors

  • Fabrice Cognasse,

    1. From the EFS Auvergne-Loire, Saint-Etienne, France; and GIMAP-EA3064, Faculty of Medicine, University of Saint-Etienne, Saint-Etienne, France.
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  • Françoise Boussoulade,

    1. From the EFS Auvergne-Loire, Saint-Etienne, France; and GIMAP-EA3064, Faculty of Medicine, University of Saint-Etienne, Saint-Etienne, France.
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  • Patricia Chavarin,

    1. From the EFS Auvergne-Loire, Saint-Etienne, France; and GIMAP-EA3064, Faculty of Medicine, University of Saint-Etienne, Saint-Etienne, France.
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  • Sophie Acquart,

    1. From the EFS Auvergne-Loire, Saint-Etienne, France; and GIMAP-EA3064, Faculty of Medicine, University of Saint-Etienne, Saint-Etienne, France.
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  • Patrick Fabrigli,

    1. From the EFS Auvergne-Loire, Saint-Etienne, France; and GIMAP-EA3064, Faculty of Medicine, University of Saint-Etienne, Saint-Etienne, France.
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  • Bernard Lamy,

    1. From the EFS Auvergne-Loire, Saint-Etienne, France; and GIMAP-EA3064, Faculty of Medicine, University of Saint-Etienne, Saint-Etienne, France.
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  • Olivier Garraud

    1. From the EFS Auvergne-Loire, Saint-Etienne, France; and GIMAP-EA3064, Faculty of Medicine, University of Saint-Etienne, Saint-Etienne, France.
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  • The study was funded in part by Baxter-Europe and by EFS Auvergne-Loire.

O. Garraud, MD, PhD, GIMAP-EA 3064, Université de Saint-Etienne, Faculté de Médecine, 15 rue Ambroise Paré, 42023 Saint-Etienne cedex 2, France; e-mail: olivier.garraud@efs.sante.fr.

Abstract

BACKGROUND:  Blood platelets (PLTs) link the processes of hemostasis and inflammation. Recent studies have demonstrated that PLTs promote immunity and inflammation mainly by means of the CD40/CD40L pathway. Our objective was to describe the accumulation of cytokines in PLT concentrates during storage.

STUDY DESIGN AND METHODS:  Pools of PLT concentrates were prepared, separated from plasma, and resuspended in clinical-grade storage medium; samples were taken on Days 0, 1, 2, 3, and 5 for analysis, without replacement (i.e., without soluble protein dilution). Interleukin (IL)-6, IL-8, PLT-derived growth factor (PDGF)-AA, soluble CD40 ligand (sCD40L), RANTES, and transforming growth factor-β production were measured by specific enzyme-linked immunosorbent assays.

RESULTS:  Over time, the levels of RANTES, IL-8, and IL-6 were stable. In contrast, the levels of PDGF-AA and sCD40L increased. Ex vivo production of sCD40L was quantified at levels sufficient to induce B-cell effects based on previous studies of in vitro induced B-cell activation and differentiation by sCD40L. Cytokine and/or chemokine levels were generally higher in PLT concentrate supernatants and/or PLT lysates in comparison to PLT-free plasma, allowing the determination of which cytokine and/or chemokine was absorbed or secreted by transfusion-grade PLTs over time.

CONCLUSION:  Our data provide evidence that stored PLTs contain molecules with known immunomodulatory competence and secrete them differentially over time during storage for transfusion purposes.

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