Prospective, paired crossover comparison of multiple, single-needle plateletpheresis procedures with the Amicus and Trima Accel cell separators

Authors

  • Stefano Fontana,

    1. From the Department of Hematology and Central Hematology Laboratory, Division of Transfusion Medicine, Inselspital, University Hospital, Bern, Switzerland.
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  • Livio Mordasini,

    1. From the Department of Hematology and Central Hematology Laboratory, Division of Transfusion Medicine, Inselspital, University Hospital, Bern, Switzerland.
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  • Peter Keller,

    1. From the Department of Hematology and Central Hematology Laboratory, Division of Transfusion Medicine, Inselspital, University Hospital, Bern, Switzerland.
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  • Behrouz Mansouri Taleghani

    1. From the Department of Hematology and Central Hematology Laboratory, Division of Transfusion Medicine, Inselspital, University Hospital, Bern, Switzerland.
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Dr Stefano Fontana, Department of Haematology and Central Haematology Laboratory, Division of Transfusion Medicine, Inselspital, University Hospital, CH-3010 Bern, Switzerland; e-mail: stefano.fontana@insel.ch.

Abstract

BACKGROUND: The Baxter Amicus Version 2.51 (A) and the Gambro BCT Trima Accel Version 5.0 (T) cell separators may produce multiple platelet (PLT) concentrates within a single donation.

STUDY DESIGN AND METHODS: The single-needle multiple plateletpheresis procedures of the two devices were compared in a prospective, randomized, paired crossover study in 60 donors. The 120 donations were compared for donor comfort, collection efficiency, residual white blood cell (WBC) count, and (in selected patients) corrected count increment (CCI).

RESULTS: The mean PLT yield and the resultant mean number of units per donation were significantly lower for A (6.06 × 1011 vs. 7.48 × 1011 and 2.57 vs. 3.19, respectively, both p < 0.001), in spite of a longer apheresis duration (89 min vs. 79 min; p < 0.001). This resulted in a higher collection rate of T (5.68 × 1011 PLTs/hr vs. 4.10 × 1011 PLTs/hr, p < 0.001). Residual WBC count of every unit was fewer than 5 × 106, but significantly fewer A-PLT donations contained more than 105 WBCs per unit (1 vs. 9, p = 0.008). Although the ACD-A consumption was slightly higher for A (489 mL vs. 469 mL, p = 0.04), a trend to a higher frequency of side effects was found for T (42.4% vs. 23.7%, p = 0.06). The 1-hour CCIs of 33 transfused A-PLT units were comparable with those of 43 T-PLT units (11.8 vs. 13.9, p = 0.480).

CONCLUSIONS: Both cell separators showed safe collections of up to 4 PLT units per donation with adequate CCI. T produced a higher PLT yield despite shorter apheresis duration, but with slightly higher residual WBC counts and a trend to a higher side-effect frequency.

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