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The effect of interruption of agitation on in vitro measures of platelet concentrates in additive solution


Pieter F. van der Meer, PhD, Sanquin Blood Bank North West Region, Plesmanlaan 125, 1066 CX, PO Box 9137, 1006 AC, Amsterdam, the Netherlands; e-mail:


BACKGROUND: Interruption of agitation results in lower in vitro quality of platelet concentrates (PCs). The rates at which the deleterious effects occur, however, are unknown. Therefore, in vitro parameters of PCs in additive solution (AS) during various periods without agitation have been investigated.

STUDY DESIGN AND METHODS: PCs from five buffy coats in AS (Composol, Fresenius HemoCare) were white cell (WBC)-reduced by filtration. Four PCs were pooled and divided to obtain paired samples. Beginning immediately after processing, three PCs were stored without agitation and placed on an agitator after 16, 20, and 24 hours. The fourth PC was agitated throughout storage and served as reference (n = 10 paired experiments).

RESULTS: pH37°C on Day 7 was greater than 6.8 in reference PCs, and in PCs that were not agitated for 16 hours, longer interruption resulted in lower pH values. During interruption of agitation, metabolic rates were significantly higher in the study groups: glucose consumption was 12.5 ± 1.6 µmol per 1011 platelets (PLTs) per hour in PCs during the first 24 hours without interruption versus 2.0 ± 0.4 µmol per 1011 PLTs per hour in the reference group (p  < 0.01). Lactate formation was 24.7 ± 4.2 versus 3.9 ± 0.4 µmol per 1011 PLTs per hour in the above-mentioned groups, respectively (p  < 0.01). Once replaced on the agitator, the metabolic rates lowered, but remained significantly elevated during consecutive storage days compared to the reference.

CONCLUSION: WBC-reduced PCs in Composol AS may experience 16 hours without agitation with no permanent effects on in vitro measures compared to reference units. During interruption of agitation, glucose and lactate metabolism is elevated, resulting in lower pH values in the subsequent storage period.