Funding for this study was provided by CaridianBCT Biotechnologies and the Stacey True Memorial Trust.
Generation of neutrophil priming activity by cell-containing blood components treated with pathogen reduction technology and stored in platelet additive solutions
Article first published online: 13 DEC 2010
© 2010 American Association of Blood Banks
Volume 51, Issue 6, pages 1220–1227, June 2011
How to Cite
Ambruso, D. R., Thurman, G., Tran, K., Marschner, S., Gathof, B., Janetzko, K. and Goodrich, R. P. (2011), Generation of neutrophil priming activity by cell-containing blood components treated with pathogen reduction technology and stored in platelet additive solutions. Transfusion, 51: 1220–1227. doi: 10.1111/j.1537-2995.2010.02983.x
- Issue published online: 9 JUN 2011
- Article first published online: 13 DEC 2010
- Received for publication July 23, 2010; revision received September 23, 2010, and accepted October 13, 2010.
BACKGROUND: Storage of cell-containing blood components such as platelet concentrates (PCs) and red blood cells (RBCs) results in generation of biologically active compounds, many of which may be associated with adverse transfusion events. Priming of the neutrophil oxidase activity is a common characteristic of many of the biologically active compounds found in stored blood. We evaluated the priming activity of pathogen reduction technology (PRT)-treated PCs stored in plasma or platelet additive solution (PAS) and PRT-treated RBCs.
STUDY DESIGN AND METHODS: PCs were collected with Trima or Amicus equipment and were PRT treated with the Mirasol PRT system or the Intercept Blood System. Some units were gamma irradiated. Products were stored in 100% plasma or 35% plasma plus PAS. RBCs were washed and PRT treated before storage. Samples were removed throughout storage and priming of the oxidase activity was measured.
RESULTS: Platelets collected on Trima or Amicus equipment and stored in plasma or PAS demonstrated increasing priming activity during 5 to 7 days of storage. Gamma irradiation, but not PRT treatment with either technology, further enhanced this priming activity. Supernatants of RBCs stored for 42 days induced priming in untreated controls, but not in washed or Mirasol PRT–treated test products.
CONCLUSION: Production of oxidase priming activity increased during storage in all blood products. No significant differences were associated with the collection method, storage in PAS, or PRT treatment. The generation of biologically active compounds, which may serve as an etiology for adverse events, appears to be independent of these processes for collection, storage, and pathogen reduction.