This research was supported by grant from National Natural Science of China (Grants 81072350 and 30872223) and the Hi-tech Research and Development (863) Program of China (2009AA02Z111).
Absence of xenotropic murine leukemia virus–related virus in blood donors in China
Article first published online: 19 AUG 2011
© 2012 American Association of Blood Banks
Volume 52, Issue 2, pages 326–331, February 2012
How to Cite
Mi, Z., Lu, Y., Zhang, S., An, X., Wang, X., Chen, B., Wang, Q. and Tong, Y. (2012), Absence of xenotropic murine leukemia virus–related virus in blood donors in China. Transfusion, 52: 326–331. doi: 10.1111/j.1537-2995.2011.03267.x
- Issue published online: 12 JAN 2012
- Article first published online: 19 AUG 2011
- Received for publication January 15, 2011; revision received April 13, 2011, and accepted June 6, 2011.
BACKGROUND: Xenotropic murine leukemia virus–related virus (XMRV) is a novel human gammaretrovirus that was first identified in patients with prostate cancer in 2006. Subsequent studies have shown that XMRV is also detected in patients with chronic fatigue syndrome (CFS) and even in some healthy controls and blood donors. However, some conflicting findings have been reported by different laboratories or in different regions. The association of XMRV with human diseases and the prevalence of XMRV in different populations needs to be further determined.
STUDY DESIGN AND METHODS: XMRV was screened in 391 blood samples from healthy blood donors in China. Nested reverse transcription–polymerase chain reaction (PCR) was used to amplify gag and env genes of XMRV from total RNA of peripheral blood mononuclear cells (PBMNCs) and plasma, respectively. Quantitative PCR was performed to detect XMRV env gene in genomic DNA of PBMNCs. To enhance the detection sensitivity, plasma was added into LNCaP cells to amplify XMRV in the plasma samples.
RESULTS: No XMRV was found in the 391 blood donors in China or in the LNCaP cells inoculated with plasma from the blood donors.
CONCLUSION: Both PCR and virus isolation in highly permissive LNCaP cells failed to detect XMRV in 391 Chinese blood donors, indicating that XMRV infection might not be present in blood donors in China.