FCGR3B*03 allele inheritance pattern in Brazilian families and some new variants of gene FCGR3B
Version of Record online: 2 SEP 2011
© 2011 American Association of Blood Banks
Volume 52, Issue 3, pages 629–634, March 2012
How to Cite
Terzian, C. C.N., Chiba, A. K., Santos, V. C., Silva, N. P. and Bordin, J. O. (2012), FCGR3B*03 allele inheritance pattern in Brazilian families and some new variants of gene FCGR3B. Transfusion, 52: 629–634. doi: 10.1111/j.1537-2995.2011.03326.x
- Issue online: 8 MAR 2012
- Version of Record online: 2 SEP 2011
- Received for publication May 20, 2011; revision received July 12, 2011; and accepted July 25, 2011.
BACKGROUND: The FCGR3B gene encoding the FcyRIIIb receptor for immunoglobulin G has three polymorphic forms known as HNA-1a, HNA-1b, and HNA-1c, encoded by the alleles FCGR3B*01, FCGR3B*02, and FCGR3B*03, respectively. It is not clear whether the inheritance of the FCGR3B*03 allele, which encodes the HNA-1c, is linked or not to the other two alleles. The objective of this study was to identify the inheritance pattern of the FCGR3B*03 allele in Brazilians.
STUDY DESIGN AND METHODS: Blood samples from nine families with at least one FCGR3B*03(+) member, totalizing 47 individuals, were studied. The presence of the FCGR3B*01, FCGR3B*02, and FCGR3B*03 alleles was detected by the polymerase chain reaction with sequence-specific priming method, and all DNA samples were sequenced.
RESULTS: In three of the nine studied families, the FCGR3B*03 was passed down with the FCGR3B*02, while in one family the FCGR3B*03 was inherited in linkage with FCGR3B*01. The other families were not informative regarding FCGR3B*03 inheritance. Sequencing showed for the first time one single-nucleotide polymorphism at Position 264 resulting from a simple substitution CT; three other different substitutions at Position 230, TA, TG; and the presence of three nucleotides at Position 230 (T, G, and A). The previously reported variants FCGR3B*01A227G and FCGR3BG330T were also found.
CONCLUSION: In this Brazilian FCGR3B*03(+) group we found that the inheritance of FCGR3B*03 took place by a linkage to FCGR3B*02 or to FCGR3B*01. Linkage of FCGR3B*03 to FCGR3B*02 was the most common. Additionally, we report SNPs that have not been described, suggesting that they might be more common than previously thought.