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Quantitative and qualitative analysis of coagulation factors in cryoprecipitate prepared from fresh-frozen plasma inactivated with amotosalen and ultraviolet A light

Authors

  • Joan Cid,

    Corresponding author
    1. From the Hemotherapy-Hemostasis Department, CDB, IDIBAPS, Hospital Clínic, University of Barcelona; and the Blood and Tissue Bank, Barcelona, Spain.
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  • Carolina Caballo,

    1. From the Hemotherapy-Hemostasis Department, CDB, IDIBAPS, Hospital Clínic, University of Barcelona; and the Blood and Tissue Bank, Barcelona, Spain.
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  • Marc Pino,

    1. From the Hemotherapy-Hemostasis Department, CDB, IDIBAPS, Hospital Clínic, University of Barcelona; and the Blood and Tissue Bank, Barcelona, Spain.
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  • Ana M. Galan,

    1. From the Hemotherapy-Hemostasis Department, CDB, IDIBAPS, Hospital Clínic, University of Barcelona; and the Blood and Tissue Bank, Barcelona, Spain.
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  • Nuria Martínez,

    1. From the Hemotherapy-Hemostasis Department, CDB, IDIBAPS, Hospital Clínic, University of Barcelona; and the Blood and Tissue Bank, Barcelona, Spain.
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  • Ginés Escolar,

    1. From the Hemotherapy-Hemostasis Department, CDB, IDIBAPS, Hospital Clínic, University of Barcelona; and the Blood and Tissue Bank, Barcelona, Spain.
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  • Maribel Diaz-Ricart

    1. From the Hemotherapy-Hemostasis Department, CDB, IDIBAPS, Hospital Clínic, University of Barcelona; and the Blood and Tissue Bank, Barcelona, Spain.
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  • This work has been partially supported by Grants PET 2008-0231, FIS PI080156 (Fondo de Investigaciones de la Seguridad Social), German Jose Carreras Leukaemia Foundation (R 07/41v), SAF2009-10365, SAF2011-28214 (Ministerio de Ciencia y Tecnología), and RD06/0009/1003 (Red HERACLES, Instituto de Salud Carlos III).

Joan Cid, MD, PhD, Servei d'Hemoteràpia i Hemostàsia, Hospital Clínic, C/. Villarroel, 170, 08036 Barcelona, Spain; e-mail: jcid@clinic.ub.es.

Abstract

BACKGROUND: There were no previous studies about the quality of cryoprecipitate prepared from fresh-frozen plasma (FFP) inactivated with amotosalen and ultraviolet A (UVA) light. The aim of this study was to analyze the quantity and quality of coagulation factors in cryoprecipitate prepared from FFP treated with amotosalen and UVA light.

STUDY DESIGN AND METHODS: FFP was obtained from whole blood donations and inactivated with amotosalen and UVA light according to the manufacturer's instructions. Fibrinogen, factor VIII (FVIII), von Willebrand factor antigen (VWF : Ag) and activity (VWF : RCo), the von Willebrand factor cleavage protease activity (ADAMTS-13), and the multimeric structure of VWF were analyzed.

RESULTS: The content of fibrinogen, FVIII, and ADAMTS-13 was lower in cryoprecipitates prepared from amotosalen-treated plasma when compared with cryoprecipitates prepared from nontreated plasma (35, 40, and 18% loss, respectively). The quantity and quality of VWF as well as VWF multimer patterns were not affected by the inactivation method.

CONCLUSION: Cryoprecipitates prepared from amotosalen-treated FFP contained significantly reduced levels of fibrinogen, FVIII, and ADAMTS-13. However, the VWF quantity and quality was well preserved.

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