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Fig. S1. Changes in lysophosphatidylcholine (LPC)-species in stored platelet concentrates (PLCs). Platelets (A), platelet concentrate extracellular vesicles (PLC-EVs) (B), and plasma (C) were analyzed for LPC-species content on the day of platelet apheresis (Day 0) and after 5 days of storage (Day 5). Graphical presentation based on absolute mean values (nmol/mg protein or nmol/ml plasma), showing increased species in red and decreased species in blue. Significant changes between Day 0 and Day 5 are shown by asterisks. Absolute amounts of LPC-species on Day 0 are shown in Supplementary Table S1.

Fig. S2. Changes in phosphatidylcholine (PC)-, phosphatidylserine (PS)-, phosphatidylethanolamine (PE)- and phosphatidylinositol (PI)-species distribution during platelet concentrate (PLC) storage. Platelets (A), PLC extracellular vesicles (PLC-EVs) (B) and plasma (C) were isolated and analyzed separately by mass spectrometry for PC-, PS-, PE- and PI-species (PI-species only for platelets and PLC-EVs) distribution on the day of platelet apheresis and after 5 days of storage under standard blood bank conditions. Graphical presentation based on absolute mean data (nmol/mg protein or nmol/ml plasma), showing increased species in red and decreased species in blue, whereby significant changes between Day 0 and Day 5 are shown as asterisks for each species. Absolute amounts of PC-, PS-, PE- and PI-species on Day 0 are shown in Supplementary Table S2.

Fig. S3. Changes in phosphatidylethanolamine plasmalogen (PLPE)-species distribution during platelet concentrate (PLC) storage. Platelets (A), platelet concentrate extracellular vesicles (PLC-EVs) (B), and plasma (C) were analyzed for PLPE content on the day of platelet apheresis (Day 0) and after 5 days of storage (Day 5). Graphical presentation based on absolute mean values (nmol/mg protein or nmol/ml plasma), showing increased species in red and decreased species in blue. Significant changes between Day 0 and Day 5 are shown by asterisks. Absolute amounts of PLPE-species on Day 0 are shown in Supplementary Table S3.

Fig. S4. Changes in phosphatidylcholine plasmalogen (PC O) species in stored platelet concentrates (PLCs). Platelets (A), platelet concentrate extracellular vesicles (PLC-EVs) (B), and plasma (C) were isolated and analyzed separately by mass spectrometry for PC O-species distribution on the day of platelet apheresis and after 5 days of storage under standard blood bank conditions. Graphical presentation based on absolute mean values (nmol/mg protein or nmol/ml plasma), showing increased species in red and decreased species in blue, whereby significant changes between Day 0 and Day 5 are shown as asterisks for each species. Absolute amounts of PC O-species on Day 0 are shown in Supplementary Table S4.

Fig. S5. Changes in cholesteryl ester (CE) species distribution in released platelet concentrate extracellular vesicles (PLC-EVs) during 5 days PLC storage. PLC-EVs were isolated by differential centrifugation on the day of PLC apheresis (PLC-EVs Day 0) and after 5 days in vitro storage (PLC-EVs Day 5) and analyzed by mass spectrometry for CE-species distribution. Absolute mean data and significant changes between Day 0 and Day 5 are shown.

Fig. S6. Changes in sphingomyelin (SM) species distribution in stored platelet concentrates (PLCs). A) Platelets and B) PLC extracellular vesicles (PLC-EVs) were isolated and analyzed separately by mass spectrometry for SM-species distribution on the day of platelet apheresis (Day 0) and after 5 days of storage (Day 5) under standard blood bank conditions. Absolute mean data (nmol/mg protein) and significant changes between Day 0 and Day 5 are shown.

Fig. S7. Changes in ceramide (Cer) species distribution in stored platelet concentrates (PLCs). Platelets and PLC extracellular vesicles (PLC-EVs) were isolated and analyzed separately by mass spectrometry for Cer- species distribution on the day of platelet apheresis (Day 0) and after 5 days of storage (Day 5) under standard blood bank conditions. Absolute mean data (nmol/mg protein) and significant changes between Day 0 and Day 5 are shown.

Table S1. Lysophosphatidylcholine (LPC)-species content (1nmol/mg or 2nmol/mL) in fresh (Day 0) platelets, platelet extracellular vesicles (PLC-EVs) and plasma. n.d. = not determined, 3under the detection limit (10−4 nmol/mg). Significant differences in respective lipid species between platelets and PLC-EVs are shown as asterisks.

Table S2. (A) Phosphatidylcholine (PC)-, (B) phosphatidylserine (PS)-, (C) phosphatidylethanolamine (PE)- and (D) phosphatidylinositol (PI)-species content (1nmol/mg or 2nmol/mL) in fresh (Day 0) platelets, platelet concentrate extracellular vesicles (PLC-EVs) and plasma. n.d. = not determined. (PI-species are only shown for platelets and PLC-EVs.)

Table S3. Phosphatidylethanolamine plasmalogen (PLPE)-species content (1nmol/mg or 2nmol/mL) in fresh (Day 0) platelets, platelet concentrate extracellular vesicles (PLC-EVs) and plasma. Significant differences in respective lipid species between platelets and PLC-EVs are shown as asterisks.

Table S4. Phosphatidylcholine plasmalogen (PC O)-species content (1nmol/mg or 2nmol/mL) in fresh (Day 0) platelets, platelet concentrate extracellular vesicles (PLC-EVs) and plasma. Significant differences in respective lipid species between platelets and PLC-EVs are shown as asterisks.

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trf3775_sm_MaterialS4.pdf67KSupporting info item
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