This research was supported by the University of California, Los Angeles, David Geffen School of Medicine, Department of Pathology and Laboratory Medicine Translational Research Fund.
Comparison of a gel microcolumn assay with the conventional tube test for red blood cell alloantibody titration
Article first published online: 13 JUL 2012
© 2012 American Association of Blood Banks
Volume 53, Issue 4, pages 811–815, April 2013
How to Cite
Finck, R., Lui-Deguzman, C., Teng, S.-M., Davis, R. and Yuan, S. (2013), Comparison of a gel microcolumn assay with the conventional tube test for red blood cell alloantibody titration. Transfusion, 53: 811–815. doi: 10.1111/j.1537-2995.2012.03793.x
- Issue published online: 8 APR 2013
- Article first published online: 13 JUL 2012
- Received for publication October 19, 2011; revision received May 30, 2012, and accepted May 31, 2012.
BACKGROUND: Titration is a semiquantitative method used to estimate red blood cell (RBC) alloantibody reactivity. The conventional tube test (CTT) technique is the traditional method for performing titration studies. The gel microcolumn assay (GMA) is also a sensitive method to detect RBC alloantibodies. The aim of this study was to compare a GMA with the CTT technique in the performance of Rh and K alloantibody titration.
STUDY DESIGN AND METHODS: Patient serum samples that contained an RBC alloantibody with a singular specificity were identified by routine blood bank workflow. Parallel titration studies were performed on these samples by both the CTT method and a GMA (ID-Micro Typing System anti-IgG gel card, Micro Typing Systems, Inc., an Ortho-Clinical Diagnostics Company).
RESULTS: Forty-eight samples were included, including 11 anti-D, five anti-c, 13 anti-E, one anti-C, three anti-e, and 15 anti-K. Overall, the two methods generated identical results in 21 of 48 samples. For 42 samples (87.5%) the two methods generated results that were within one serial dilution, and for the remaining six samples, results were within two dilutions.
CONCLUSION: GMA systems may perform comparably to the CTT in titrating alloantibodies to Rh and Kell antigens.