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Transfusion of banked red blood cells and the effects on hemorrheology and microvascular hemodynamics in anemic hematology outpatients

Authors

  • Koray Yürük,

    1. From the Department of Translational Physiology and the Department of Clinical Hematology, Academic Medical Center, University of Amsterdam, Amsterdam, the Netherlands; and the Department of Intensive Care, Erasmus MC, University Medical Center, Rotterdam, the Netherlands.
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  • Dan M.J. Milstein,

    1. From the Department of Translational Physiology and the Department of Clinical Hematology, Academic Medical Center, University of Amsterdam, Amsterdam, the Netherlands; and the Department of Intensive Care, Erasmus MC, University Medical Center, Rotterdam, the Netherlands.
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  • Rick Bezemer,

    Corresponding author
    1. From the Department of Translational Physiology and the Department of Clinical Hematology, Academic Medical Center, University of Amsterdam, Amsterdam, the Netherlands; and the Department of Intensive Care, Erasmus MC, University Medical Center, Rotterdam, the Netherlands.
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  • Sebastiaan A. Bartels,

    1. From the Department of Translational Physiology and the Department of Clinical Hematology, Academic Medical Center, University of Amsterdam, Amsterdam, the Netherlands; and the Department of Intensive Care, Erasmus MC, University Medical Center, Rotterdam, the Netherlands.
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  • Bart J. Biemond,

    1. From the Department of Translational Physiology and the Department of Clinical Hematology, Academic Medical Center, University of Amsterdam, Amsterdam, the Netherlands; and the Department of Intensive Care, Erasmus MC, University Medical Center, Rotterdam, the Netherlands.
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  • Can Ince

    1. From the Department of Translational Physiology and the Department of Clinical Hematology, Academic Medical Center, University of Amsterdam, Amsterdam, the Netherlands; and the Department of Intensive Care, Erasmus MC, University Medical Center, Rotterdam, the Netherlands.
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  • This study has been financially supported by the Landsteiner Foundation for Blood Research (Grant 2006-0621).

Rick Bezemer, PhD, Department of Translational Physiology, Academic Medical Center, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands; e-mail: R.Bezemer@amc.uva.nl.

Abstract

BACKGROUND: The aim of this study was to investigate the effects of red blood cell (RBC) transfusion on the hemorrheologic properties and microcirculatory hemodynamics in anemic hematology outpatients receiving 2 to 4 RBC units of either “fresh” (leukoreduced storage for less than 1 week) or “aged” (leukoreduced storage for 3-4 weeks) RBCs.

STUDY DESIGN AND METHODS: Measurements were performed before and 30 minutes after RBC transfusion in hematology outpatients. Leukoreduced RBC suspensions were stored in saline-adenine-glucose-mannitol (SAGM) additive solution. Whole blood viscosity was measured using Couette low-shear viscometry, RBC deformability and aggregability were measured using laser-assisted optical rotational cell analysis, and microcirculatory density and perfusion were assessed using sidestream dark field imaging.

RESULTS: One group of patients (n = 10) received a median (interquartile range) of 3 (2-3) RBC bags that were stored for 7 (5-7) days (fresh) and the other group of patients (n = 10) received 3 (3-3) RBC bags that were stored for 23 (22-28) days (aged). After transfusion of fresh versus aged RBCs, hematocrit increased to 32 ± 3% versus 31 ± 2% (p < 0.363), whole blood viscosity increased to 4.2 ± 0.4 Pa/sec versus 4.2 ± 0.6 Pa/sec (p < 0.912), RBC deformability index remained unaffected, RBC aggregability index increased to 55 ± 10 versus 55 ± 13 (p = 0.967), microcirculatory flow remained unaffected, and microcirculatory density increased to 19.3 ± 2.5 mm/mm2 versus 18.7 ± 1.9 mm/mm2 (p = 0.595), respectively.

CONCLUSION: Storing leukoreduced SAGM-suspended RBCs for 3 to 4 weeks did not affect their ability to improve hemorrheologic properties and microcirculatory hemodynamics in our small group of anemic hematology outpatients. Larger studies are needed to confirm this finding.

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