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A prospective study on the efficacy of mobilization of autologous peripheral stem cells in pediatric oncohematology patients

Authors

  • Simone Cesaro,

    1. Pediatric Hematology Oncology, Azienda Ospedaliera Universitaria Integrata, Verona, Italy
    2. Pediatric Hematology Oncology, Ospedale Pediatrico Meyer, Firenze, Italy
    3. Pediatric Hematology Oncology, Ospedale Regina Margherita, Torino, Italy
    4. Pediatric Oncology, Istituto Nazionale Tumori, Milan, Italy
    5. Pediatric Hematology Oncology, Department of Pediatrics, Padova, Italy
    6. Pediatric Hematology Oncology, Istituto G. Gaslini, Genova, Italy
    7. Pediatric Hematology Oncology, Ospedale San Gerardo, Monza, Italy
    8. Pediatric Hematology Oncology, Ospedale Silvestrini, Perugia, Italy
    9. Pediatric Oncology, Ospedale San Giovanni Rotondo, Foggia, Italy
    10. Pediatric Hematology Oncology, Ospedale Lalla Seragnoli, Bologna, Italy
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  • Veronica Tintori,

    1. Pediatric Hematology Oncology, Azienda Ospedaliera Universitaria Integrata, Verona, Italy
    2. Pediatric Hematology Oncology, Ospedale Pediatrico Meyer, Firenze, Italy
    3. Pediatric Hematology Oncology, Ospedale Regina Margherita, Torino, Italy
    4. Pediatric Oncology, Istituto Nazionale Tumori, Milan, Italy
    5. Pediatric Hematology Oncology, Department of Pediatrics, Padova, Italy
    6. Pediatric Hematology Oncology, Istituto G. Gaslini, Genova, Italy
    7. Pediatric Hematology Oncology, Ospedale San Gerardo, Monza, Italy
    8. Pediatric Hematology Oncology, Ospedale Silvestrini, Perugia, Italy
    9. Pediatric Oncology, Ospedale San Giovanni Rotondo, Foggia, Italy
    10. Pediatric Hematology Oncology, Ospedale Lalla Seragnoli, Bologna, Italy
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  • Francesca Nesi,

    1. Pediatric Hematology Oncology, Azienda Ospedaliera Universitaria Integrata, Verona, Italy
    2. Pediatric Hematology Oncology, Ospedale Pediatrico Meyer, Firenze, Italy
    3. Pediatric Hematology Oncology, Ospedale Regina Margherita, Torino, Italy
    4. Pediatric Oncology, Istituto Nazionale Tumori, Milan, Italy
    5. Pediatric Hematology Oncology, Department of Pediatrics, Padova, Italy
    6. Pediatric Hematology Oncology, Istituto G. Gaslini, Genova, Italy
    7. Pediatric Hematology Oncology, Ospedale San Gerardo, Monza, Italy
    8. Pediatric Hematology Oncology, Ospedale Silvestrini, Perugia, Italy
    9. Pediatric Oncology, Ospedale San Giovanni Rotondo, Foggia, Italy
    10. Pediatric Hematology Oncology, Ospedale Lalla Seragnoli, Bologna, Italy
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  • Elisabetta Schiavello,

    1. Pediatric Hematology Oncology, Azienda Ospedaliera Universitaria Integrata, Verona, Italy
    2. Pediatric Hematology Oncology, Ospedale Pediatrico Meyer, Firenze, Italy
    3. Pediatric Hematology Oncology, Ospedale Regina Margherita, Torino, Italy
    4. Pediatric Oncology, Istituto Nazionale Tumori, Milan, Italy
    5. Pediatric Hematology Oncology, Department of Pediatrics, Padova, Italy
    6. Pediatric Hematology Oncology, Istituto G. Gaslini, Genova, Italy
    7. Pediatric Hematology Oncology, Ospedale San Gerardo, Monza, Italy
    8. Pediatric Hematology Oncology, Ospedale Silvestrini, Perugia, Italy
    9. Pediatric Oncology, Ospedale San Giovanni Rotondo, Foggia, Italy
    10. Pediatric Hematology Oncology, Ospedale Lalla Seragnoli, Bologna, Italy
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  • Elisabetta Calore,

    1. Pediatric Hematology Oncology, Azienda Ospedaliera Universitaria Integrata, Verona, Italy
    2. Pediatric Hematology Oncology, Ospedale Pediatrico Meyer, Firenze, Italy
    3. Pediatric Hematology Oncology, Ospedale Regina Margherita, Torino, Italy
    4. Pediatric Oncology, Istituto Nazionale Tumori, Milan, Italy
    5. Pediatric Hematology Oncology, Department of Pediatrics, Padova, Italy
    6. Pediatric Hematology Oncology, Istituto G. Gaslini, Genova, Italy
    7. Pediatric Hematology Oncology, Ospedale San Gerardo, Monza, Italy
    8. Pediatric Hematology Oncology, Ospedale Silvestrini, Perugia, Italy
    9. Pediatric Oncology, Ospedale San Giovanni Rotondo, Foggia, Italy
    10. Pediatric Hematology Oncology, Ospedale Lalla Seragnoli, Bologna, Italy
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  • Sandro Dallorso,

    1. Pediatric Hematology Oncology, Azienda Ospedaliera Universitaria Integrata, Verona, Italy
    2. Pediatric Hematology Oncology, Ospedale Pediatrico Meyer, Firenze, Italy
    3. Pediatric Hematology Oncology, Ospedale Regina Margherita, Torino, Italy
    4. Pediatric Oncology, Istituto Nazionale Tumori, Milan, Italy
    5. Pediatric Hematology Oncology, Department of Pediatrics, Padova, Italy
    6. Pediatric Hematology Oncology, Istituto G. Gaslini, Genova, Italy
    7. Pediatric Hematology Oncology, Ospedale San Gerardo, Monza, Italy
    8. Pediatric Hematology Oncology, Ospedale Silvestrini, Perugia, Italy
    9. Pediatric Oncology, Ospedale San Giovanni Rotondo, Foggia, Italy
    10. Pediatric Hematology Oncology, Ospedale Lalla Seragnoli, Bologna, Italy
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  • Maddalena Migliavacca,

    1. Pediatric Hematology Oncology, Azienda Ospedaliera Universitaria Integrata, Verona, Italy
    2. Pediatric Hematology Oncology, Ospedale Pediatrico Meyer, Firenze, Italy
    3. Pediatric Hematology Oncology, Ospedale Regina Margherita, Torino, Italy
    4. Pediatric Oncology, Istituto Nazionale Tumori, Milan, Italy
    5. Pediatric Hematology Oncology, Department of Pediatrics, Padova, Italy
    6. Pediatric Hematology Oncology, Istituto G. Gaslini, Genova, Italy
    7. Pediatric Hematology Oncology, Ospedale San Gerardo, Monza, Italy
    8. Pediatric Hematology Oncology, Ospedale Silvestrini, Perugia, Italy
    9. Pediatric Oncology, Ospedale San Giovanni Rotondo, Foggia, Italy
    10. Pediatric Hematology Oncology, Ospedale Lalla Seragnoli, Bologna, Italy
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  • Ilaria Capolsini,

    1. Pediatric Hematology Oncology, Azienda Ospedaliera Universitaria Integrata, Verona, Italy
    2. Pediatric Hematology Oncology, Ospedale Pediatrico Meyer, Firenze, Italy
    3. Pediatric Hematology Oncology, Ospedale Regina Margherita, Torino, Italy
    4. Pediatric Oncology, Istituto Nazionale Tumori, Milan, Italy
    5. Pediatric Hematology Oncology, Department of Pediatrics, Padova, Italy
    6. Pediatric Hematology Oncology, Istituto G. Gaslini, Genova, Italy
    7. Pediatric Hematology Oncology, Ospedale San Gerardo, Monza, Italy
    8. Pediatric Hematology Oncology, Ospedale Silvestrini, Perugia, Italy
    9. Pediatric Oncology, Ospedale San Giovanni Rotondo, Foggia, Italy
    10. Pediatric Hematology Oncology, Ospedale Lalla Seragnoli, Bologna, Italy
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  • Raffaella Desantis,

    1. Pediatric Hematology Oncology, Azienda Ospedaliera Universitaria Integrata, Verona, Italy
    2. Pediatric Hematology Oncology, Ospedale Pediatrico Meyer, Firenze, Italy
    3. Pediatric Hematology Oncology, Ospedale Regina Margherita, Torino, Italy
    4. Pediatric Oncology, Istituto Nazionale Tumori, Milan, Italy
    5. Pediatric Hematology Oncology, Department of Pediatrics, Padova, Italy
    6. Pediatric Hematology Oncology, Istituto G. Gaslini, Genova, Italy
    7. Pediatric Hematology Oncology, Ospedale San Gerardo, Monza, Italy
    8. Pediatric Hematology Oncology, Ospedale Silvestrini, Perugia, Italy
    9. Pediatric Oncology, Ospedale San Giovanni Rotondo, Foggia, Italy
    10. Pediatric Hematology Oncology, Ospedale Lalla Seragnoli, Bologna, Italy
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  • Desirèe Caselli,

    1. Pediatric Hematology Oncology, Azienda Ospedaliera Universitaria Integrata, Verona, Italy
    2. Pediatric Hematology Oncology, Ospedale Pediatrico Meyer, Firenze, Italy
    3. Pediatric Hematology Oncology, Ospedale Regina Margherita, Torino, Italy
    4. Pediatric Oncology, Istituto Nazionale Tumori, Milan, Italy
    5. Pediatric Hematology Oncology, Department of Pediatrics, Padova, Italy
    6. Pediatric Hematology Oncology, Istituto G. Gaslini, Genova, Italy
    7. Pediatric Hematology Oncology, Ospedale San Gerardo, Monza, Italy
    8. Pediatric Hematology Oncology, Ospedale Silvestrini, Perugia, Italy
    9. Pediatric Oncology, Ospedale San Giovanni Rotondo, Foggia, Italy
    10. Pediatric Hematology Oncology, Ospedale Lalla Seragnoli, Bologna, Italy
    Search for more papers by this author
  • Franca Fagioli,

    1. Pediatric Hematology Oncology, Azienda Ospedaliera Universitaria Integrata, Verona, Italy
    2. Pediatric Hematology Oncology, Ospedale Pediatrico Meyer, Firenze, Italy
    3. Pediatric Hematology Oncology, Ospedale Regina Margherita, Torino, Italy
    4. Pediatric Oncology, Istituto Nazionale Tumori, Milan, Italy
    5. Pediatric Hematology Oncology, Department of Pediatrics, Padova, Italy
    6. Pediatric Hematology Oncology, Istituto G. Gaslini, Genova, Italy
    7. Pediatric Hematology Oncology, Ospedale San Gerardo, Monza, Italy
    8. Pediatric Hematology Oncology, Ospedale Silvestrini, Perugia, Italy
    9. Pediatric Oncology, Ospedale San Giovanni Rotondo, Foggia, Italy
    10. Pediatric Hematology Oncology, Ospedale Lalla Seragnoli, Bologna, Italy
    Search for more papers by this author
  • Roberto Luksch,

    1. Pediatric Hematology Oncology, Azienda Ospedaliera Universitaria Integrata, Verona, Italy
    2. Pediatric Hematology Oncology, Ospedale Pediatrico Meyer, Firenze, Italy
    3. Pediatric Hematology Oncology, Ospedale Regina Margherita, Torino, Italy
    4. Pediatric Oncology, Istituto Nazionale Tumori, Milan, Italy
    5. Pediatric Hematology Oncology, Department of Pediatrics, Padova, Italy
    6. Pediatric Hematology Oncology, Istituto G. Gaslini, Genova, Italy
    7. Pediatric Hematology Oncology, Ospedale San Gerardo, Monza, Italy
    8. Pediatric Hematology Oncology, Ospedale Silvestrini, Perugia, Italy
    9. Pediatric Oncology, Ospedale San Giovanni Rotondo, Foggia, Italy
    10. Pediatric Hematology Oncology, Ospedale Lalla Seragnoli, Bologna, Italy
    Search for more papers by this author
  • Irene Panizzolo,

    1. Pediatric Hematology Oncology, Azienda Ospedaliera Universitaria Integrata, Verona, Italy
    2. Pediatric Hematology Oncology, Ospedale Pediatrico Meyer, Firenze, Italy
    3. Pediatric Hematology Oncology, Ospedale Regina Margherita, Torino, Italy
    4. Pediatric Oncology, Istituto Nazionale Tumori, Milan, Italy
    5. Pediatric Hematology Oncology, Department of Pediatrics, Padova, Italy
    6. Pediatric Hematology Oncology, Istituto G. Gaslini, Genova, Italy
    7. Pediatric Hematology Oncology, Ospedale San Gerardo, Monza, Italy
    8. Pediatric Hematology Oncology, Ospedale Silvestrini, Perugia, Italy
    9. Pediatric Oncology, Ospedale San Giovanni Rotondo, Foggia, Italy
    10. Pediatric Hematology Oncology, Ospedale Lalla Seragnoli, Bologna, Italy
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  • Gloria Tridello,

    1. Pediatric Hematology Oncology, Azienda Ospedaliera Universitaria Integrata, Verona, Italy
    2. Pediatric Hematology Oncology, Ospedale Pediatrico Meyer, Firenze, Italy
    3. Pediatric Hematology Oncology, Ospedale Regina Margherita, Torino, Italy
    4. Pediatric Oncology, Istituto Nazionale Tumori, Milan, Italy
    5. Pediatric Hematology Oncology, Department of Pediatrics, Padova, Italy
    6. Pediatric Hematology Oncology, Istituto G. Gaslini, Genova, Italy
    7. Pediatric Hematology Oncology, Ospedale San Gerardo, Monza, Italy
    8. Pediatric Hematology Oncology, Ospedale Silvestrini, Perugia, Italy
    9. Pediatric Oncology, Ospedale San Giovanni Rotondo, Foggia, Italy
    10. Pediatric Hematology Oncology, Ospedale Lalla Seragnoli, Bologna, Italy
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  • Arcangelo Prete

    1. Pediatric Hematology Oncology, Azienda Ospedaliera Universitaria Integrata, Verona, Italy
    2. Pediatric Hematology Oncology, Ospedale Pediatrico Meyer, Firenze, Italy
    3. Pediatric Hematology Oncology, Ospedale Regina Margherita, Torino, Italy
    4. Pediatric Oncology, Istituto Nazionale Tumori, Milan, Italy
    5. Pediatric Hematology Oncology, Department of Pediatrics, Padova, Italy
    6. Pediatric Hematology Oncology, Istituto G. Gaslini, Genova, Italy
    7. Pediatric Hematology Oncology, Ospedale San Gerardo, Monza, Italy
    8. Pediatric Hematology Oncology, Ospedale Silvestrini, Perugia, Italy
    9. Pediatric Oncology, Ospedale San Giovanni Rotondo, Foggia, Italy
    10. Pediatric Hematology Oncology, Ospedale Lalla Seragnoli, Bologna, Italy
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Address reprint requests to: Simone Cesaro, MD, Pediatric Hematology Oncology, Policlinico G.B. Rossi, Azienda Ospedaliera Universitaria Integrata, P.le L.A. Scuro, 10, 37134 Verona, Italy; e-mail: simone.cesaro@ospedaleuniverona.it.

Abstract

Background

Peripheral blood stem cells (PBSCs) are the preferred source in autologous transplantation. We assessed prospectively the efficacy of mobilization in pediatric patients and risk factors associated with its failure.

Study Design and Methods

Patients, aged 0 to 17 years, needing a first collection of PBSCs for autologous stem cell transplantation were eligible. The study period was from July 2008 to September 2010. A blood peak of fewer than 20 × 106 CD34+ cells/L was used as the cutoff to define a poor mobilizer.

Results

A total of 145 patients, 57% male (82) and 43% female (63), with a median age of 7 years, affected by solid tumor, 79% (114), and acute leukemia or lymphoma, 21% (31), were enrolled. Granulocyte–colony-stimulating factor used was filgrastim in 69%, lenograstim in 26%, and pegfilgrastim in 5% of patients. A total of 83% (121) of patients mobilized successfully, the median CD34+ count being 120 × 106/L (range, 23 × 106-1840 × 106/L). A single leukapheresis procedure was sufficient to achieve the target CD34+ cell dose in 82% (99/121) of patients. Among 24 poor mobilizer patients, 15 underwent a second mobilizing course and nine required a marrow harvest. Factors associated with poor mobilization were metastatic disease and relapse. Among 99 patients who underwent autologous stem cell transplantation, the median times to neutrophil and platelet engraftment and of hospitalization were longer by 2, 12, and 6 days in poor versus good mobilizer group.

Conclusions

In pediatric patients undergoing a first mobilization, the incidence of poor mobilization was 17%. Failure of mobilization resulted in an increase in health costs and a longer hospitalization for those who underwent autologous stem cell transplantation.

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