This work was partially supported by a grant from the San Antonio Life Sciences Institute.
Enhanced shear-induced platelet aggregation due to low-temperature storage
Version of Record online: 9 OCT 2012
© 2012 American Association of Blood Banks
Volume 53, Issue 7, pages 1520–1530, July 2013
How to Cite
Montgomery, R. K., Reddoch, K. M., Evani, S. J., Cap, A. P. and Ramasubramanian, A. K. (2013), Enhanced shear-induced platelet aggregation due to low-temperature storage. Transfusion, 53: 1520–1530. doi: 10.1111/j.1537-2995.2012.03917.x
- Issue online: 11 JUL 2013
- Version of Record online: 9 OCT 2012
- Manuscript Accepted: 22 AUG 2012
- Manuscript Revised: 8 AUG 2012
- Manuscript Received: 30 MAY 2012
- San Antonio Life Sciences Institute
Refrigeration of platelets (PLTs) offers an attractive alternative to the currently practiced storage at room temperature since it may mitigate problems associated with bacterial contamination and extend storage lifetime. Refrigeration causes a number of biophysical and biochemical changes in PLTs and decreases PLT circulation time in vivo. However, the effect of refrigeration on PLT hemostatic functions under physiologic and pathophysiologic shear conditions has not been adequately characterized.
Study Design and Methods
Washed PLTs prepared from either fresh PLT-rich plasma (PRP) or PRP stored at 4°C for 2 days was mixed with exogenous von Willebrand factor (VWF) and fibrinogen and sheared in a cone-and-plate viscometer. PLT aggregation, activation, and VWF binding after shear and glycoprotein (GP) Ibα receptor expression and ristocetin-induced PLT agglutination were measured.
PLTs stored at 4°C for 2 days aggregated significantly more than fresh PLTs particularly at high shear rates (10,000/sec), and this increase was independent of PLT concentration or suspension viscosity. Further, refrigerated PLTs showed a greater increase in GP Ibα–dependent PLT activation under shear and also bound more VWF than fresh PLTs. However, the GP Ibα expression levels as measured by three different antibodies were significantly lower in refrigerated PLTs than in fresh PLTs, and refrigeration resulted in a modest decrease in ristocetin-induced PLT agglutination.
The combined results demonstrate that refrigeration increases PLT aggregation under high shear, but not static, conditions and also increases shear-induced VWF binding and PLT activation. Clinically, enhanced shear-induced PLT aggregation due to low temperature storage may be a beneficial strategy to prevent severe bleeding in trauma.