1These authors contributed equally to this manuscript.
Characterization of endothelial-like cells derived from human mesenchymal stem cells
Article first published online: 9 JAN 2007
Journal of Thrombosis and Haemostasis
Volume 5, Issue 4, pages 826–834, April 2007
How to Cite
LIU, J. W., DUNOYER-GEINDRE, S., SERRE-BEINIER, V., MAI, G., LAMBERT, J.-F., FISH, R. J., PERNOD, G., BUEHLER, L., BOUNAMEAUX, H. and KRUITHOF, E. K. O. (2007), Characterization of endothelial-like cells derived from human mesenchymal stem cells. Journal of Thrombosis and Haemostasis, 5: 826–834. doi: 10.1111/j.1538-7836.2007.02381.x
- Issue published online: 2 APR 2007
- Article first published online: 9 JAN 2007
- Received 27 March 2006, accepted 28 December 2006
- endothelial cell;
- mesenchymal stem cells;
Summary. Background: Blood-derived endothelial progenitor cells (EPC) have been used to treat ischemic disease. However, the number of EPC that can be obtained from adult blood is limited.
Objective: To characterize endothelial-like cells obtained from human bone marrow and determine their ability to stimulate new blood vessel formation in vivo.
Methods: Mononuclear cells (MNC) were isolated from human bone marrow or umbilical cord blood and cultured in endothelial growth medium (EGM-2). Mesenchymal stem cells (MSC) were isolated from bone marrow and induced to differentiate into endothelial-like cells (MSCE), or adipocytes or osteocytes by growth in EGM-2, adipogenic or osteogenic medium.
Results: Cells obtained by culturing bone marrow MNC in EGM-2 formed cord- or tube-like structures when grown on MatrigelTM and expressed several endothelial marker proteins. However, cell morphology and the profile of endothelial marker protein expression were different from those of cord blood-derived EPC (cbEPC). Cells with a similar phenotype were obtained by differentiation of MSC into MSCE, which was accompanied by an increase of endothelial marker proteins and a diminished capacity to differentiate into adipocytes. Subcutaneous implantation of MSCE in collagen plugs in non-obese diabetic-severe combined immunodeficient (NOD-SCID) mice resulted in formation of functional blood vessels that had incorporated the MSCE.
Conclusions: Our results show that MSCE and cbEPC are different cell types. The formation of functional blood vessels by MSCE, combined with high yields and a reduced capacity to differentiate into other cell types compared with MSC, makes these cells potentially useful for autologous therapy of ischemic disease.