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Figure  S1. Genetic background does not alter platelet deposition response on collagen or stability during shear step-up. Platelet accumulation over patterned collagen for 300 s at an average wall shear rate of 400 s−1 for both black (C57B1/6) and white (BalbC/ByJ) wild-type mice, followed by a shear step-up test of stability at 8000 s−1. Each data point is an average of six mice and error bars represent standard deviations.

Figure  S2. Mouse platelet deposition at 100 s−1 on collagen reaches steady state within 400 s. Time traces of platelet accumulation on patterned collagen as a function of shear and time. For an = 100 s−1, there was no net increase in platelet accumulation after 5 min. Data are presented as the mean (n ≥ 6) and the error bars represent the standard deviation.

Figure  S3. Schematic of photomask used to for lithography. The white area outside the flow channels forms a vacuum groove in the final devices that is accessed by a vacuum tube (located at a position seen in the lower right corner of each mask). Black bars between the flow channels provide for stabilizing struts within the vacuum groove.

Figure  S4. Photograph of finished silicon master wafer used for casting.

Figure  S5. Schematic of the microfluidic protein patterning protocol used. Please refer to Supplemental section entitled ‘Collagen patterning protocol’ for details.

Video  SM1. Representative video of wild-type black (C57BL/6) mouse platelets perfused at a volumetric flow rate of 0.64 μL min−1 ( = 100 s−1,  = 126 s−1).

Video  SM2. Representative video of wild-type black (C57BL/6) mouse platelets perfused at a volumetric flow rate of 2.5 μL min−1 ( = 400 s−1,  = 492 s−1).

Video  SM3. Representative video of wild-type black (C57BL/6) mouse platelets perfused at a volumetric flow rate of 6.4 μL min−1 (= 1000 s−1, = 1260 s−1).

Video  SM4. Representative video of α2−/− mouse platelets perfused at a volumetric flow rate of 2.5 μL min−1 (= 400 s−1, = 492 s−1).

Video  SM5. Representative video of α2−/−/FcRγ−/− mouse platelets perfused at a volumetric flow rate of 2.5 μL min−1 (= 400 s−1, = 492 s−1).

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JTH_3188_sm_figures1.eps453KSupporting info item
JTH_3188_sm_figures2.eps450KSupporting info item
JTH_3188_sm_figures3.eps1681KSupporting info item
JTH_3188_sm_figures4.tif2947KSupporting info item
JTH_3188_sm_figures5.eps299KSupporting info item
JTH_3188_sm_supplement.doc48KSupporting info item
JTH_3188_sm_video sm1.mov5116KSupporting info item
JTH_3188_sm_video sm2.mov16936KSupporting info item
JTH_3188_sm_video sm3.mov4264KSupporting info item
JTH_3188_sm_video sm4.mov2678KSupporting info item
JTH_3188_sm_video sm5.mov2568KSupporting info item

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.