This work has been presented as an oral communication at the XXIst Conference of the International Society on Thrombosis and Haemostasis in Boston, MA, USA (July 2009).
Mechanisms underlying FeCl3-induced arterial thrombosis
Version of Record online: 4 APR 2011
© 2011 International Society on Thrombosis and Haemostasis
Journal of Thrombosis and Haemostasis
Volume 9, Issue 4, pages 779–789, April 2011
How to Cite
ECKLY, A., HECHLER, B., FREUND, M., ZERR, M., CAZENAVE, J.-P., LANZA, F., MANGIN, P. H. and GACHET, C. (2011), Mechanisms underlying FeCl3-induced arterial thrombosis. Journal of Thrombosis and Haemostasis, 9: 779–789. doi: 10.1111/j.1538-7836.2011.04218.x
- Issue online: 4 APR 2011
- Version of Record online: 4 APR 2011
- Accepted manuscript online: 24 JAN 2011 08:11AM EST
- Received 26 July 2010, accepted 7 January 2011
- carotid artery;
- ferric chloride;
- tissue factor;
- vascular injury
See also Brill A. A ride with ferric chloride. This issue, pp 776–8.
Summary. Background: The FeCl3-induced vascular injury model is widely used to study thrombogenesis in vivo, but the processes leading to vascular injury and thrombosis are poorly defined. Objectives: The aim of our study was to better characterize the mechanisms of FeCl3-induced vascular injury and thrombus formation, in order to evaluate the pathophysiological relevance of this model. Methods: FeCl3 was applied at different concentrations (from 7.5% to 20%) and for different time periods (up to 5 min) to mouse carotid or mesenteric arteries. Results: Under all the conditions tested, ultrastructural analysis revealed that FeCl3 diffused through the vessel wall, resulting in endothelial cell denudation without exposure of the inner layers. Hence, only the basement membrane components were exposed to circulating blood cells and might have contributed to thrombus formation. Shortly after FeCl3 application, numerous ferric ion-filled spherical bodies appeared on the endothelial cells. Interestingly, platelets could adhere to these spheres and form aggregates. Immunogold labeling revealed important amounts of tissue factor at their surface, suggesting that these spheres may play a role in thrombin generation. Invitro experiments indicated that FeCl3 altered the ability of adhesive proteins, including collagen, fibrinogen and von Willebrand factor, to support platelet adhesion. Finally, real-time intravital microscopy showed no protection against thrombosis in GPVI-immunodepleted and β1−/− mice, suggesting that GPVI and β1 integrins, known to be involved in initial platelet adhesion and activation, do not play a critical role in FeCl3-induced thrombus formation. Conclusion: This model should be used cautiously, in particular to study the earliest stage of thrombus formation.