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Keywords:

  • inside-out regulation;
  • integrin;
  • integrin activation;
  • kindlin;
  • talin

Abstract

  1. Top of page
  2. Abstract
  3. Disrupting the transmembrane domain (TMD) interaction between α and β subunits induces integrin activation
  4. Talin binding to the integrin β tail disrupts the TMD interaction
  5. Kindlin is an important regulator of integrin
  6. Other signalling molecules that may contribute to integrin activation
  7. Future developments
  8. Disclosure of Conflict of Interest
  9. References

Summary.  Integrins are cell surface adhesion and signaling receptors important for cell adhesion, survival and migration. Integrins are known to be regulated by signals from inside the cells. Such inside-out regulation modulates affinities of integrins for their extracellular matrix ligand and is critical for thrombosis, haemostasis and immune response. Talin and kindlin, two integrin binding proteins, have been shown to be important regulators of integrin function. In this review, we will focus on the molecular mechanism of integrin regulation that has emerged from recent structural, biochemical and genetic studies.

Loss of endothelial layer in blood vessel by injury exposes subendothelial extracellular cellular matrix proteins such as collagen, platelets subsequently adhere to collagen-bound von Willebrand factor and other matrix components [1,2]. Upon platelet adhesion, thromboxane A2 is synthesised from arachidonic acid by cyclooxygenase-1 in platelets and is released. In addition, granules containing various agonists including ADP are released [3]. Platelet agonists are also released from injured cells or generated by the proteolysis during coagulation [4]. These molecules bind to their receptors on platelet surfaces and induce intracellular signalling resulting in platelet aggregation, critical events in achieving haemostasis to prevent further bleeding at the site of the injury. A platelet adhesion molecule integrin αIIbβ3, a heterodimeric type I transmembrane protein consisting of α and β subunit, is a key component of the platelet aggregation [5]. When activated by the intracellular signalling, the integrin become highly adhesive toward its ligand fibrinogen in the circulating blood stream. Since the fibrinogen has multiple binding sites for the integrin, their interaction can crosslink platelets to facilitate aggregation. In this way, integrin αIIbβ3 plays a vital role in haemostasis.

Proper integrin regulation prevents integrin αIIbβ3 from engaging their ligand and thus avoids the formation of intravascular thrombi under normal conditions. Inappropriate platelet aggregation, usually on vessels involved by atherosclerosis, can be dangerous. Uncontrolled formation of thrombus can block blood flow and cause tissue infarction. Therefore, the affinity of integrin αIIbβ3 is tightly regulated so that the integrin is kept in low affinity state (inactive) and enters a high affinity state (active) only when and where needed. Many structural studies suggest that there are at least two different conformations of extracellular domain corresponding to each affinity state [6–9]. The integrin seems to be inactive when the extracellular domain is folded as V-shape with the ligand-binding headpiece facing the membrane (Fig. 1A), while it becomes highly adhesive toward its ligand when the extracellular domain is extended (Fig. 1B). These conformational changes can be induced by intracellular signalling, so called ‘inside-out’ signalling [10]. In this review, we will describe how the inside-out signalling can be transmitted to extracellular domain to make such conformational changes in extracellular domain, as well as many intracellular molecular players involved in the signalling. This review will focus on our current understanding, and we apologise for any omissions that this focus engenders.

image

Figure 1.  Structural model of proposed integrin αIIbβ3 affinity states. (A) In resting state, α and β TMDs interact with each other, and the extracellular domains folded with the ligand-binding site facing cell membrane. (B) In high affinity state, α and β TMDs are separated, and the extracellular domains are extended.

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Disrupting the transmembrane domain (TMD) interaction between α and β subunits induces integrin activation

  1. Top of page
  2. Abstract
  3. Disrupting the transmembrane domain (TMD) interaction between α and β subunits induces integrin activation
  4. Talin binding to the integrin β tail disrupts the TMD interaction
  5. Kindlin is an important regulator of integrin
  6. Other signalling molecules that may contribute to integrin activation
  7. Future developments
  8. Disclosure of Conflict of Interest
  9. References

An earlier mutational study suggested that there is an electrostatic interaction in the membrane-proximal region of cytoplasmic domain (Fig. 2A), between Arg995 of αIIb and Asp723 of β3 [11]. Charge reversal (e.g. αIIb [R995D], β3 [D723R]) of either residue induces integrin activation but combined charge reversals of both residues makes the integrin inactive again. This observation led to a hypothesis that an interaction between cytoplasmic tails of integrin αIIb and β3 subunits can keep the integrin inactive and breaking the interaction can activate integrins. In addition to the charged residues, many other activating mutations are also found in the membrane proximal regions. For example, mutating two Phe in the membrane proximal region of αIIb (Fig. 2A) into Ala makes the integrin constitutively active [11]. Since the membrane proximal regions of integrins contain unusual hydrophobic patches which may be embedded in the membrane together with the transmembrane domain (Fig. 2A), those activating effects induced by mutations in the regions were interpreted that membrane embedding of those membrane proximal region may be important for regulating integrin affinity [12].

image

Figure 2.  Integrin αIIbβ3 TMD structure. (A) Amino acid sequences of integrin αIIbβ3 around TMD. TMD regions are highlighted in yellow box. Amino acid residues mentioned in the text are indicated in red. The conventional TMD regions and the membrane proximal regions are indicated with blue solid and dotted lines, respectively. (B) NMR structure of integrin αIIb (red) and β3 (blue) (PDB entry 2k9j) is shown. The amino acid residues indicated in (A) are shown as stick models. (C) The αβ TMD interaction through GXXXG motif, or outer membrane clasp, is highlighted. Surface of αIIb TMD is shown with glycines in the GXXXG motif indicated as yellow. (D) The αβ TMD interaction through the Asp723–Arg995, or inner membrane clasp, is highlighted. Surface of β3 TMD with the Asp723 indicated as red.

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Activating mutations of integrin αIIbβ3 are also found in transmembrane domains (TMDs). Interestingly, most of the activating mutations found were substitutions of Gly resides in both αIIb (Gly972, Gly976) and β3 TMDs (Gly708) with amino acids containing bulky side chain, such as Leu, Ile, or Asn [13–15]. Especially, two Gly in αIIb TMD are part of a GXXXG motif, a motif important in TMD helix–helix association [16] in other transmembrane proteins. Therefore, the α-β TMD packing guided by a groove formed by those Gly was also considered important to keep the integrin inactive. However, this finding is not consistent with other previous mutational and structural studies. For example, TMDs and membrane proximal regions of both αIIb and β3 were considered to form continuous helices [17] and the packing of TMDs via those Gly residues makes TMDs to cross each other [18]. The crossing angle between the TMDs, predicted by the structural studies [18], does not permit the known αIIb Arg995–β3 Asp723 electrostatic interaction (at least 10 Å apart in the structure). Nevertheless, mutation either in the αIIb GXXXG motif or in the αIIb or β3 membrane proximal regions disrupts the αIIbβ3 TMD-interaction [19], suggesting simultaneous formation of both the TMD packing (later termed as outer membrane clasp or OMC) and the membrane-proximal region interaction (later termed as inner membrane clasp or IMC) are required to maintain an inactive integrin. Therefore, a structural explanation of these activating mutations is of great interest.

Recently, the NMR structure of integrin αIIbβ3 TMD complex embedded in lipid bilayer was solved (Fig. 2B) [20]. The structure shows that αIIb and β3 TMDs pack together via the Gly residues with a vertical αIIb TMD and tilted β3 TMD, which forms the OMC (Fig. 2C). β3 TMD and the membrane proximal region make a continuous helix as expected. The hydrophobic amino acid patch in the β3 membrane-proximal region is inserted into membrane as a part of TMD and makes long and tilted helix. In contrast, the αIIb TMD makes a short and straight helix that ends at Gly991. The membrane proximal region next to the Gly991 turns toward β3, which enables the two Phe residues (Phe992 and Phe993) outside of the transmembrane helix to be embedded into membrane (Fig. 2D) generating this unusual transmembrane domain. In addition, the turning of the membrane proximal region places the Arg995 residue close to Asp723 of β3, thus allowing formation of IMC (Fig. 2D). Thus, the structure of αIIbβ3 TMD complex, explains how all the mutations discussed above can activate integrin.

Talin binding to the integrin β tail disrupts the TMD interaction

  1. Top of page
  2. Abstract
  3. Disrupting the transmembrane domain (TMD) interaction between α and β subunits induces integrin activation
  4. Talin binding to the integrin β tail disrupts the TMD interaction
  5. Kindlin is an important regulator of integrin
  6. Other signalling molecules that may contribute to integrin activation
  7. Future developments
  8. Disclosure of Conflict of Interest
  9. References

Talin, a 270 kDa cytoplasmic protein, can bind to integrin β tails through its 50 kDa head domain [21,22] and has multiple actin binding sites in its 220 kDa rod domain [11,23,24]. Over-expression of the integrin-binding head domain in cells strongly activates β3 [21], β1 [25] and β2 integrins [26,27]. Genetic ablation of talin is embryonic lethal due to defects in cell migration at gastrulation [28]. Tissue specific deletion of talin in cells of the megakaryocyte lineage caused severe defects in platelet aggregation and agonist-stimulated integrin activation. Consequently mice that have lost talin in megakaryocytes and platelets have prolonged bleeding time and experience frequent pathological bleeding [29,30]. Conditional knockout of talin in macrophages result in defective phagocytosis and β2 integrin mediated adhesion [26]. Thus talin is an important regulator of integrin activation.

The talin head domain contains a FERM domain, (band 4.1, Ezrin, Radixin, and Moesin homology domain), which is usually further divided into F1, F2 and F3 subdomains [31]. The talin head has an extra N-terminal F0 subdomain [32]. The integrin binding PTB (phospho-tyrosine binding) domain is located within F3 and is sufficient to activate β3 integrin [33]. What features in talin made it unique in its ability to activate integrin amongst the FERM domain containing or PTB domain containing proteins? Compared to other FERM domain proteins, talin has two integrin binding sites: a strong binding site to the β3-W739DTANNPLY747 sequence involving mainly the S5 strand of the F3 domain and a hydrophobic pocket formed by R358, A360, and Y377 of F3 (membrane distal [MD] interaction) [34,35], and a weak binding site to the β3-H722DRKEFAKFEEER726 involving the loop between S1 and S2 strand (membrane proximal [MP] interaction) [34]. This weak interaction between talin F3 and the integrin MP interaction site depends upon the presence of the strong talin-integrin MD interaction. In addition to two integrin binding sites, the talin F2 and F3 domain also have multiple positively charged residues on its surface that can interact with the negatively charged phospholipids and potentially favour the right configuration of talin-integrin interaction [34,36]. A patch of basic residues in talin F1 domain has also been identified [32]. A recent crystal structure of talin head domain revealed a novel extended arrangement of talin F0, F1, F2 and F3 domains, which is different from the typical compact cloverleaf structure observed in most FERM domains [37]. Thus, the unique extended structure of the talin FERM domain allows simultaneous contact of the basic residues on F1, F2, and F3 with lipids [37].

Since we have detailed understanding about the structural mechanism of how integrin activated by disruption of TM and cytoplasmic domain interactions, the obvious question to ask is how these unique features of talin-integrin interactions lead to disruption of integrin cytoplasmic and TM interactions? Talin is recruited to the plasma membrane by intracellular signals such as Rap1 and RIAM (Rap1 interacting adaptor molecule) [38–41]. At the plasma membrane, talin will then engage with β integrin cytoplasmic tails. Whether talin is actively recruited to the integrin β tail by some signalling events or it simply encounters integrin through an efficient two-dimensional diffusion once at the plasma membrane is still unclear. Upon engaging with integrin, talin interacts with β integrin cytoplasmic tail through its strong MD binding site. Any mutation to integrin or talin that interrupts this strong site interaction would abolish integrin activation by talin [34,35]. Following this strong site interaction, the weak talin-β tail MP interaction and talin-membrane interaction occurs. These sequential interactions are supported by the observation that talin can still bind to soluble β integrin cytoplasmic tail even when the weak interaction site is disrupted in a system where the membrane is absent [34]. Whether the weak talin-β3 MP interaction and the talin-membrane interaction occur sequentially or simultaneously is not clear.

The engagement of talin with plasma membrane and with two β integrin cytoplasmic tail binding sites as well as the restricted topology of β transmembrane and cytoplasmic domain by lipid bilayer probably puts the talin-β integrin complex in a precise configuration poised to trigger the activation of integrin. Mutations in β integrin or in talin that disrupt the weak talin-β cytoplasmic interaction site have small or no effects on the affinity of talin for the β integrin cytoplasmic tail but markedly impair integrin activation [34]. Platelets expressing talin with the same point mutation that disrupts talin-integrin β3 MP weak interaction site have defects in integrin activation and platelet aggregation [42]. Mutations in F1, F2 or F3 that interrupt with the talin-lipid interaction also impaired integrin activation by talin [32,34,36]. The final definitive proof is provided by an in vitro reconstitution of inside-out activation with purified talin head and integrin nanodiscs. The talin head is sufficient to activate integrins in a purified system only when interactions with membrane and two talin binding sites in β integrin cytoplasmic tail are present [9]. Thus, the requirement for the multiple interactions between talin, integrin and membrane is well established by multiple lines of work.

The more interesting question though, is how this configuration of talin, integrin and membrane can trigger integrin activation. There are several possible mechanisms. The first one is that talin causes a motion, possibly tilting, pistonning or lateral motion in integrin β transmembrane and cytoplasmic domain, which disrupt integrin α and β subunit interactions at both IMC and OMC. This hypothesis is supported by a recent molecular dynamics simulation which showed that talin can change the tilting angle of integrin β subunit [43]. Since the tilting angle is important to maintain optimal α and β subunit IMC and OMC, a tilting angle change would disrupt these interactions leading to integrin activation [19,20]. The second hypothesis is that this precise configuration brings talin Lys324 close to Asp723 of the integrin β3 subunit, disrupting the Asp–Arg (R995-D723 in αIIbβ3) salt bridge, thus favouring integrin activation. This hypothesis is supported by the observation of talin Lys-integrin β Asp interaction in a crystal structure of talin F2F3–β1D complex [36]. The third hypothesis is that talin might come in between integrin α and β subunit, acting like a wedge to open the integrin α and β TM and cytoplasmic clasps through steric hindrance. This idea is illustrated in Fig. 3. When the structure of the talin head-integrin α and β TM complex is assembled by aligning talin head and α and β TM complex with talin F2F3–β1D complex, we observed a possible clash between talin head and integrin α tails. These three possible mechanisms are not mutually exclusive and further research will be needed to pinpoint the exact mechanism.

image

Figure 3.  Binding of talin to β3 tail might result in steric clash between talin and αIIb. Two αIIbβ3 TMD structure was depicted in the figure. Shown in red and blue are the integrin αII and β3 TMD from the recent NMR structure (PDB entry 2k9j) [20]. Shown in orange and cyan are the αIIb and β3 TMD from the recent Rosetta structure [62]. Talin head domain (PDB entry 3IVF) was shown in magenta. The structure of the whole complex was assembled by first aligning a β1D-Talin F2F3 (PDB entry 3G9W. β1D cytoplasmic domain in green) complex structure to the β3, and then aligning the talin head structure to the talin F2F3 (not shown) structure. Membrane distal (MD) and membrane proximal (MP) interactions were indicated. Talin head overlaps with αIIb in the Rosetta structure, indicating potential steric hindrance.

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Kindlin is an important regulator of integrin

  1. Top of page
  2. Abstract
  3. Disrupting the transmembrane domain (TMD) interaction between α and β subunits induces integrin activation
  4. Talin binding to the integrin β tail disrupts the TMD interaction
  5. Kindlin is an important regulator of integrin
  6. Other signalling molecules that may contribute to integrin activation
  7. Future developments
  8. Disclosure of Conflict of Interest
  9. References

Recently, another family of integrin binding proteins, kindlins, has been shown to be important for proper integrin regulation. There are three kindlin orthologues in mammals: kindlin-1, -2 and -3. Genetic ablation of kindlin-2 or -3 are embryonic lethal in mice [44,45]. Loss of functional kindlin-3 in platelets results in severe bleeding, defective platelet aggregation and platelet integrin activation [44]. Similarly, defects in β2 integrin activation and the integrin-mediated adhesion were reported in leukocytes that have lost functional kindlin-3 [46,47]. Knock out of kindlin-1 and 2 inhibited β1 integrin activation and resulted in defective cell attachment [45,48]. In humans, loss of functional kindlin-3 has been shown to be the cause of a subset of leukocyte adhesion deficiency III (LAD III, LAD Iv) patients [47,49,50]. Mutations and truncations in kindlin-1 are the cause of Kindler’s Syndrome [48,51]. Thus, the importance of kindlin in integrin regulation is well supported by the current literature.

Compared to the detailed mechanistic understanding of integrin activation by talin, we are still at the beginning stage of understanding the mechanism of integrin regulation by kindlin. Kindlin has a FERM domain. But the F2 subdomain is separated into two halves by a PH domain in the middle. Deletion of the PH domain reduced kindlin’s capacity to synergise with talin head in activating integrin αIIbβ3 [52]. Given the usual property of PH domain as a lipid bilayer interacting partner, this result would indicate that the contact between kindlin and membrane might also plays a role in kindlin function. The consensus from the current literature is that kindlin binds to the membrane distal NPxY motif while talin binds to the membrane proximal NPxY motif [44,52,53]. Similar to talin, kindlin binds to integrin through its F3 subdomain [44]. What structural features gave kindlin the specificity to the membrane distal rather than the membrane proximal NPxY motif is not clear. Mutations in kindlin or integrin β tail that disrupt kindlin-integrin interaction inhibited the capacity of kindlin to synergise with talin head in integrin activation [52,53]. Thus, the interaction between kindlin and integrin is required for kindlin function, at least for its function to synergise with talin head in activating αIIbβ3. However, rather than synergising with the talin head in activation α5β1 integrin, over-expression of kindlins inhibited the capacity of talin head to activate α5β1 [53]. This observation is incompatible with the simple mechanism that kindlin and talin bind to integrin β tail and co-activate integrins. Instead, a hypothesis that kindlin might function as a scaffold has been proposed. A couple of recent reviews summarised possible mechanisms of kindlin function [10,54].

Other signalling molecules that may contribute to integrin activation

  1. Top of page
  2. Abstract
  3. Disrupting the transmembrane domain (TMD) interaction between α and β subunits induces integrin activation
  4. Talin binding to the integrin β tail disrupts the TMD interaction
  5. Kindlin is an important regulator of integrin
  6. Other signalling molecules that may contribute to integrin activation
  7. Future developments
  8. Disclosure of Conflict of Interest
  9. References

Talin and kindlin are the two most well documented integrin regulators, supported by data from genetically engineered mice, human patients, biochemical studies, structural studies and cell biological studies. Other molecules have also been implicated in the regulation of integrin, most notably integrin linked kinas (ILK). Conditional knock-out of ILK in platelets caused modest defects in platelet integrin activation and platelet aggregation [55]. Loss of ILK expression in CHO cells inhibited activation of a chimeric integrin αIIbα6Bβ3, which is constitutively active but sensitive to cytoplasmic integrin regulators [56]. Current literature sheds very little light on the mechanism for ILK function in integrin regulation. ILK was reported to interact with integrins by some labs [57], but others have not been able to detect the interaction [58]. ILK also displays interactions with kindlin in yeast-2-hybrid system [59]. Thus it is possible that ILK function in integrin regulation is associated with kindlin function. Another kindlin binding protein, migfilin, can weakly activate integrin when over-expressed in cells. Migfilin was proposed to exhibit its integrin activation effect by displacing a known integrin inhibitor, filamin [60]. However, genetic ablation of migfilin in mice produced no obvious phenotype and had no effects on cell adhesion, cell spreading, and integrin activation [61]. The question then is whether there are any paralogues of migfilin that might have compensated its function.

Future developments

  1. Top of page
  2. Abstract
  3. Disrupting the transmembrane domain (TMD) interaction between α and β subunits induces integrin activation
  4. Talin binding to the integrin β tail disrupts the TMD interaction
  5. Kindlin is an important regulator of integrin
  6. Other signalling molecules that may contribute to integrin activation
  7. Future developments
  8. Disclosure of Conflict of Interest
  9. References

The regulation of integrin is important for haemostasis, proper leukocyte function and endothelial cell function. Thus, this continues to be an area of intense interest. In the past several years, remarkable progress in identifying the key players and understanding their mechanism of action has been achieved. Nevertheless, important unanswered questions, such as the mechanism whereby kindlins support integrin activation, remain. The significance of integrin activation in thrombosis and inflammation also suggests that this extraordinary progress in the basic understanding of this process may lead to ‘translational moments’ in which new therapies can emerge.

References

  1. Top of page
  2. Abstract
  3. Disrupting the transmembrane domain (TMD) interaction between α and β subunits induces integrin activation
  4. Talin binding to the integrin β tail disrupts the TMD interaction
  5. Kindlin is an important regulator of integrin
  6. Other signalling molecules that may contribute to integrin activation
  7. Future developments
  8. Disclosure of Conflict of Interest
  9. References