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A Model of Exposure to Rotavirus from Nondietary Ingestion Iterated by Simulated Intermittent Contacts

Authors

  • Timothy R. Julian,

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      Department of Civil and Environmental Engineering, Stanford University, Stanford, CA 94305-4020, USA.

  • Robert A. Canales,

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      Department of Civil and Environmental Engineering, Stanford University, Stanford, CA 94305-4020, USA.

  • James O. Leckie,

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      Department of Civil and Environmental Engineering, Stanford University, Stanford, CA 94305-4020, USA.

  • Alexandria B. Boehm

    Corresponding author
      *Address correspondence to Alexandria B. Boehm, 473 Via Ortega, Y2E2 189, Stanford, CA 94305, USA; tel: (650)-724-9128; fax: (650)-725-3164; aboehm@stanford.edu.
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      Department of Civil and Environmental Engineering, Stanford University, Stanford, CA 94305-4020, USA.


*Address correspondence to Alexandria B. Boehm, 473 Via Ortega, Y2E2 189, Stanford, CA 94305, USA; tel: (650)-724-9128; fax: (650)-725-3164; aboehm@stanford.edu.

Abstract

Existing microbial risk assessment models rarely incorporate detailed descriptions of human interaction with fomites. We develop a stochastic-mechanistic model of exposure to rotavirus from nondietary ingestion iterated by simulated intermittent fomes-mouth, hand-mouth, and hand-fomes contacts typical of a child under six years of age. This exposure is subsequently translated to risk using a simple static dose-response relationship. Through laboratory experiments, we quantified the mean rate of inactivation for MS2 phage on glass (0.0052/hr) and mean transfer between fingertips and glass (36%). Simulations using these parameters demonstrated that a child's ingested dose from a rotavirus-contaminated ball ranges from 2 to 1,000 virus over a period of one hour, with a median value of 42 virus. These results were heavily influenced by selected values of model parameters, most notably the concentration of rotavirus on fomes, frequency of fomes-mouth contacts, frequency of hand-mouth contacts, and virus transferred from fomes to mouth. The model demonstrated that mouthing of fomes is the primary exposure route, with hand mouthing contributions accounting for less than one-fifth of the child's dose over the first 10 minutes of interaction.

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