This investigation received support from the UNDP/WORLD BANK/WHO Special Program for Research and Training in Tropical Diseases, and from Research contract AID/DSP E-C-0036, United States Agency for International Development.
Separation and Concentration of Schizonts of Plasmodium falciparum by Percoll Gradients1
Article first published online: 30 APR 2007
The Journal of Protozoology
Volume 30, Issue 2, pages 367–370, May 1983
How to Cite
RIVADENEIRA, E. M., WASSERMAN, M. and ESPINAL, C. T. (1983), Separation and Concentration of Schizonts of Plasmodium falciparum by Percoll Gradients. The Journal of Protozoology, 30: 367–370. doi: 10.1111/j.1550-7408.1983.tb02932.x
- Issue published online: 30 APR 2007
- Article first published online: 30 APR 2007
A technique for the separation of schizonts of Plasmodium falciparum is described. The different stages of the asexual cell cycle of the parasite were positioned according to their density in a continuous gradient of Percoll. Young trophozoites coincided with erythrocytes in a broad band corresponding to densities from 1.075 to 1.100 g/ml, whereas schizonts were concentrated at a density approximating 1.062 g/ml. The viability of the parasites was unimpaired by this procedure. Young trophozoites and schizonts continued their normal life cycle when cultured after the separation procedure. The percentage of recovery was high, reaching 80% of the initial quantity. Possible applications of the technique are discussed.