Three-Dimensional Reconstruction of Rabbit-Derived Pneumocystis carinii from Serial-Thin Sections I: Trophozoite

  1. FRANCOIS PALLUAULT1,
  2. BRUNO PIETRZYK2,
  3. EDUARDO DEI-CAS1,3,
  4. CHRISTIAN SLOMIANNY1,
  5. BENOIT SOULEZ1,3,
  6. DANIEL CAMUS1,3

Article first published online: 1 MAY 2007

DOI: 10.1111/j.1550-7408.1991.tb01377.x

Issue

Journal of Eukaryotic Microbiology

Journal of Eukaryotic Microbiology

Volume 38, Issue 4, pages 402–407, July 1991

Additional Information

How to Cite

PALLUAULT, F., PIETRZYK, B., DEI-CAS, E., SLOMIANNY, C., SOULEZ, B. and CAMUS, D. (1991), Three-Dimensional Reconstruction of Rabbit-Derived Pneumocystis carinii from Serial-Thin Sections I: Trophozoite. Journal of Eukaryotic Microbiology, 38: 402–407. doi: 10.1111/j.1550-7408.1991.tb01377.x

Author Information

  1. 1

    I.N.S.E.R.M. (U. 42), Unité de Biologie et Biochimie Parasitaires et Fongiques, domaine du C.E.R.T.I.A., 369, rue Jules-Guesde, Flers-Bourg, 59650 Villeneuve d'Ascq, France

  2. 2

    Hautes Etudes Industrielles, 13, rue de Toul, 59800 Lille, France

  3. 3

    Laboratoire de Parasitologie-Mycologie, Faculté de Médecine, 1, Place de Verdun, 59045 Lille, France

Publication History

  1. Issue published online: 1 MAY 2007
  2. Article first published online: 1 MAY 2007
  3. Received 12-17-90; accepted 4-22-91

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Keywords:

  • Computer-aided reconstruction;
  • endomembrane system;
  • ultrastructure.

The highly complex ultrastructural morphology of the endomembrane system in Pneumocystis carinii led us to perform three-dimensional reconstruction from serial-thin sections using the CATIA (Conception Assistée Tridimensionnelle Inter Active) Dassault system program. The three-dimensional reconstruction of a small trophozoite made it possible to better understand the morphological relationship among organelles and to suggest cytophysiological hypotheses. By reconstructing other parasite stages, we gathered information about the evolution of organelles during the life cycle and about their physiology.

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