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Phylogenetic Analysis of the Small Subunit Ribosomal RNA of Marteilia.refringens Validates the Existence of Phylum Paramyxea (Desportes and Perkins, 1990)1

Authors

  • FRANCK C. J. BERTHE,

    Corresponding author
    1. Lahoratoire de Génétique et Pathologic, IFREMER, BP 133 Ronce-Les-Bains, 17390 La Tremblade, France
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  • FRÉDÉRIQUE LE ROUX,

    1. Lahoratoire de Génétique et Pathologic, IFREMER, BP 133 Ronce-Les-Bains, 17390 La Tremblade, France
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  • ERIC PEYRETAILLADE,

    1. Laboraloire de Protislologie moléculaire et cellulaire des parasites opportunistes, LBCP, UPRESA CNRS 6023, Université Blaise Pascal, 63177 Aubière, France
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  • PIERRE PEYRET,

    1. Laboraloire de Protislologie moléculaire et cellulaire des parasites opportunistes, LBCP, UPRESA CNRS 6023, Université Blaise Pascal, 63177 Aubière, France
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  • DAVID RODRIGUEZ,

    1. Lahoratoire de Génétique et Pathologic, IFREMER, BP 133 Ronce-Les-Bains, 17390 La Tremblade, France
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  • MANOLO GOUY,

    1. Laboratoire de Riomélrie et Biologie Evolutive, UMR CNRS 5558, Université Claude Bernard-Lyon 1, 69622 Villeurkanne cedex, France
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  • CHRISTIAN P. VIVARÈS

    1. Laboraloire de Protislologie moléculaire et cellulaire des parasites opportunistes, LBCP, UPRESA CNRS 6023, Université Blaise Pascal, 63177 Aubière, France
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    Accession number: AJ250699

Corresponding Author: F. Berthe—Telephone number: 33 5 46 36 98 36; Fax number: 33 5 46 36 37 52; Email: fberthe@ifremer.fr

Abstract

ABSTRACT. Marteilia refringens is recognized as one of the most significant pathogens of bivalve molluscs. The nucleotide sequence of the small subunit ribosomal RNA gene of Marteilia refringens is used to elucidate the phylogenetic position of the phylum Paramyxea. Genomic DNA was extracted from sporangia of Marteilia, purified from infected blue mussels, Mytilus edulis, and flat oysters, Ostrea edulis. The sequences obtained from Marteilia species purified from both oysters and mussels were identical. The sequence identity was confirmed by in situ hybridization using a DNA probe targeted to a variable region of the ribosomal DNA. The small subunit ribosomal RNA gene sequence of M. refringens is very different from all known sequences of eukaryotic organisms, including those of myxosporeans and haplosporeans. Therefore, the phylum Paramyxea should continue to be recognized as an independent eukaryotic phylum.

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