SWP25, A Novel Protein Associated with the Nosema bombycis Endospore

Authors

  • ZHENGLI WU,

    1. The Key Sericultural Laboratory of Agricultural Ministry, Southwest University, Chongqing 400716, China, and
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    • 2Present address: Department of Biochemistry, School of Biological Science and Technology, Central South University, Changsha, Hunan 410013, China.

  • YANHONG LI,

    1. The Key Sericultural Laboratory of Agricultural Ministry, Southwest University, Chongqing 400716, China, and
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  • GUOQING PAN,

    1. The Key Sericultural Laboratory of Agricultural Ministry, Southwest University, Chongqing 400716, China, and
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  • ZEYANG ZHOU,

    1. The Key Sericultural Laboratory of Agricultural Ministry, Southwest University, Chongqing 400716, China, and
    2. Laboratory of Animal Biology, Chongqing Normal University, Chongqing 400047, China
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  • ZHONGHUAI XIANG

    1. The Key Sericultural Laboratory of Agricultural Ministry, Southwest University, Chongqing 400716, China, and
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  • 1Nucleotide sequence of the SWP25 determined in the present study is deposited in GenBank with the Accession number EF683102.

Corresponding Author: Prof. Zeyang Zhou, The Key Sericultural Laboratory of Agricultural Ministry, Southwest University, Beibei, Chongqing 400716, China—Telephone number: +86 23 68251088; FAX number: +86 23 68251128; e-mail: zyzhou@swu.edu.cn

Abstract

ABSTRACT. Microsporidia are eukaryotic, obligate intracellular, spore-forming parasites. The resistant spores, which harbor a rigid cell wall, are critical for their host-to-host transmission and persistence in the environment. The spore wall comprises two major layers: the exospore and the endospore. In Nosema bombycis, two spore wall proteins have been characterized—an endosporal protein, SWP30, and an exosporal protein, SWP32. Here, we report the identification of the third spore wall protein of N. bombycis, SWP25, the gene of which has no known homologue. SWP25 is predicted to posses a signal peptide and a heparin-binding motif. Immunoelectron microscopy analysis showed that this protein is localized to the endospore. This characterization of a new spore wall protein of N. bombycis may facilitate our investigation of the relationship between N. bombycis and its host, Bombyx mori.

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