In vitro studies are carried out on a phosphate-based glass system with different compositions (45P2O5–30CaO–(25−x) Na2O–xTiO2) of TiO2 (0≤x1 mol%). The optimization of bone-bonding ability of prepared glass samples is carried out by using studies such as ultrasonic velocities, energy-dispersive X-ray spectra, X-ray diffraction (XRD), in vitro, pH, Fourier transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM). The result that is observed indicates a possible change in the structural property of glasses due to the addition of TiO2. The strong absorption band at 1100 cm−1 in FTIR pattern in all compositions that is observed indicates the presence of Ca2P2O7 after immersion in simulated body fluid solution. A high degree of absorption is noticed in 1.0 mol% of glass sample to which TiO2 was added, which ensures the growth of a rich Ca–P crystalline layer on the glass surface during in vitro studies. The XRD study on 1.0 mol% TiO2 after in vitro study confirms the formation of crystalline hydroxyapatite (HAp). SEM micrographs, FTIR, and pH observations show a small amount of formation of HAp in all prepared glass samples whereas a higher formation of HAp is noted on glass samples with 1.0 mol% TiO2.