A coating of Ti–C:H was investigated for potential applications as artificial joint materials. Herein, we conducted experimental study using fluid shear method and tribolgoical study to evaluate and analyze the adhesive strength of proteins on Ti–C:H coatings and Ti6Al4V. Sample surfaces were worn against a steel bearing (E52100) under the lubrication of egg white protein solution. The following wear track and debris analysis helped further understand their wear mechanism. Research results showed that Ti–C:H coatings on silicon substrate had a porous topography that enhanced its adhesion with the protein. The friction coefficient and wear rate for Ti–C:H was much lower than Ti6Al4V. This can be attributed to the chemical inertness and functional diamond-like features of Ti–C:H coatings enhancement in wear resistance, making it superior to Ti6Al4V.