Paper presented at Bangalore INDIA BIO 2011 Conference.
Drug-Ceramic 2-Dimensional Nanoassemblies for Drug Delivery System in Physiological Condition
Article first published online: 8 MAY 2012
© 2012 The American Ceramic Society
Journal of the American Ceramic Society
Special Issue: BIO 2011
Volume 95, Issue 9, pages 2758–2765, September 2012
How to Cite
Choi, G., Kim, S. Y., Oh, J.-M., Choy, J.-H. (2012), Drug-Ceramic 2-Dimensional Nanoassemblies for Drug Delivery System in Physiological Condition. Journal of the American Ceramic Society, 95: 2758–2765. doi: 10.1111/j.1551-2916.2012.05219.x
- Issue published online: 3 SEP 2012
- Article first published online: 8 MAY 2012
- Manuscript Accepted: 19 MAR 2012
- Manuscript Received: 1 OCT 2011
- National Research Foundation of Korea
- Ministry of knowledge Economy
The anticancer drug methotrexate (MTX) was successfully incorporated into the layered ceramic nanoparticles of layered double hydroxides (LDHs). The prepared MTX-LDH two-dimensional nanoassemblies showed enhanced chemical and thermal stability of intercalated MTX molecules through the effective electrostatic interaction with ceramic LDH layers. And the surface charge of MTX-LDHs was determined to be highly positive whereas that of MTX is fairly negative. The anticancer effect of MTX-LDH compared with MTX was evaluated in the human osteosarcoma cell culture line SaOS-2, showing much faster drug efficacy with low concentration. The time-dependent cellular uptake of MTX determined by high performance liquid chromatography showed the much enhanced cellular drug concentration of MTX-LDH treated cancer cells than the MTX treated ones. The particle size of MTX-LDH nanoassemblies was estimated in various solutions such as saline, phosphate saline, and RPMI 1640 cell culture medium, showing that the hydrodynamic size maintains at ~100 nm, regardless of the suspension media. The positive surface charge and ~100 nm size of MTX-LDHs is considered to be the major factor in the high cellular uptake and enhanced drug efficacy.