Histopathologic Evaluation of the Therapeutic Efficacy of Water and Milk Dilution for Esophageal Acid Injury
Article first published online: 29 SEP 2008
© 1995 Society for Academic Emergency Medicine
Academic Emergency Medicine
Volume 2, Issue 7, pages 587–591, July 1995
How to Cite
Homan, C. S., Maitra, S. R., Lane, B. P., Thode, H. C. and Davidson, L. (1995), Histopathologic Evaluation of the Therapeutic Efficacy of Water and Milk Dilution for Esophageal Acid Injury. Academic Emergency Medicine, 2: 587–591. doi: 10.1111/j.1553-2712.1995.tb03594.x
- Issue published online: 29 SEP 2008
- Article first published online: 29 SEP 2008
- Received; May 27, 1994 Revision received: September 9, 1994 Accepted: September 10, 1994
Objective: To determine whether acid-induced injury to the esophagus is decreased by early dilutional therapy with water or milk.
Methods: A controlled in-vitro animal model for acid injury to the esophagus was carried out using esophagi harvested from 70 Sprague-Dawley rats of both sexes and weighing 250–350 g. One control and six experimental groups each containing ten esophagi were instilled with 1 mL of 0.5 normal solution of hydrogen chloride (N HCl). Dilution with water or milk was performed at 0, 5, or 30 minutes postinjury in the experimental groups. No dilution was performed with the control group. Specimens were maintained in an oxygenated saline bath for a 60-minute experimental period and then fixed in 10% formalin for histologic evaluation. Injury severity was rated by blinded histopathologic examination using scores of 0 (no injury), 1 (minor), 2 (moderate), and 3 (severe) for the histopathologic categories: cornified epithelial cells (CEs), granular cells (GCs), granular cell nuclei (GNs), and basal cells (BCs). Red blood cells were scored as positive or negative for lysis.
Results: The controls showed the most severe outcomes. Significant differences in injury occurred for all time periods and histopathologic categories, except for the GN/water and BC/milk histopathologic category/treatment groups. However, a linear trend analysis was significant for all histopathologic categories except BC. These analyses support decreased injury in the earlier treated groups. Injury severity was highest in the most superficial cell layer (CE).
Conclusions: Emergency therapy with water or milk reduces acute acid injury to the esophagus. Earlier treatment is associated with decreased injury severity. This research supports the use of dilutional therapy with water or milk for acute acid injury to the esophagus.