Minisequencing-Based Genotyping of Duffy and ABO Blood Groups for Forensic Purposes
Article first published online: 8 MAR 2006
Journal of Forensic Sciences
Volume 51, Issue 2, pages 357–360, March 2006
How to Cite
Ferri, G., Bini, C., Ceccardi, S., Ingravallo, F., Lugaresi, F. and Pelotti, S. (2006), Minisequencing-Based Genotyping of Duffy and ABO Blood Groups for Forensic Purposes. Journal of Forensic Sciences, 51: 357–360. doi: 10.1111/j.1556-4029.2006.00058.x
- Issue published online: 8 MAR 2006
- Article first published online: 8 MAR 2006
- Received 22 June 2005; and in revised form 6 Sept. 2005; accepted 10 Oct. 2005; published 9 Feb. 2006.
- forensic science;
- single nucleotide polymorphisms;
- duffy blood group;
- ABO blood group;
- forensic application
ABSTRACT: Duffy and ABO blood group genetic polymorphisms were studied by minisequencing analysis of single-nucleotide polymorphisms (SNPs) at nucleotide positions—33, 125, 265, and 298 of the Duffy gene and at nucleotide positions—261, 297, 467, 646, and 703 of the ABO gene. In an Italian population sample, we found four alleles and seven genotypes for the Duffy and six alleles and 16 genotypes for the ABO systems. The lower limit for reproducible results was 200 pg DNA, with a range of up to 10 ng and an optimum at 1 ng. All of the 16 analyzed inclusive paternity tests were also consistent with parentage and two out of four inconsistencies with parentage cases were excluded by one or more SNPs. Although Duffy and ABO SNP typing show lower informativeness than most current forensic tests, their robustness, the limited population distribution of FY*Fy type, and the sensitivity of the minisequencing technology suggest that these markers can be useful in selected forensic applications.