Applicability of Two Commercially Available Kits for Forensic Identification of Saliva Stains

Authors

  • Benjamin C. M. Pang Ph.D.,

    1. Forensic Science Division, Hong Kong Government Laboratory, Homantin Government Offices, 88 Chung Hau Street, Kowloon, Hong Kong SAR, China.
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  • Bobbie K. K. Cheung M.Phil.

    1. Forensic Science Division, Hong Kong Government Laboratory, Homantin Government Offices, 88 Chung Hau Street, Kowloon, Hong Kong SAR, China.
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Additional information and reprint requests:
Benjamin C. M. Pang, Ph.D.
Chemist
Forensic Science Division
Hong Kong Government Laboratory
Homantin Government Offices
88 Chung Hau Street
Kowloon, Hong Kong SAR
China
E-mail: cmpang@govtlab.gov.hk

Abstract

Abstract:  The RSID-saliva test and the SALIgAE-saliva test are two recently developed forensic saliva detection kits. In this study, we compared the sensitivity and the specificity of the two test kits with the Phadebas® amylase test by analyzing amylases from various sources including human, animals, plants, and micro-organism. The data demonstrate that the RSID-saliva test and the SALIgAE-saliva test offer higher sensitivity and specificity for the detection of saliva than the Phadebas® amylase test. The detection limits of the RSID-saliva test, the SALIgAE-saliva test, and the Phadebas® amylase test equate to 10, 4, and 1000 nL, respectively for human saliva. The RSID-saliva test and the SALIgAE-saliva test were further evaluated by analyzing semen, vaginal secretion, breast milk, blood, urine, sweat, and feces. The results of the two tests are in good agreement. The two tests reacted with urine, breast milk, and feces, but not with semen, vaginal secretion, blood, and sweat.

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