TECHNICAL NOTE TOXICOLOGY
A Quantitative Method for Simultaneous Determination of 5-Methoxy-N,N-Diisopropyltryptamine and its Metabolites in Urine Using Liquid Chromatography–Electrospray lonization–Tandem Mass Spectrometry
Article first published online: 21 MAR 2011
© 2011 American Academy of Forensic Sciences
Journal of Forensic Sciences
Volume 56, Issue 4, pages 1044–1048, July 2011
How to Cite
Jin, M. J., Jin, C., Kim, J. Y., In, M. K., Kwon, O. S. and Yoo, H. H. (2011), A Quantitative Method for Simultaneous Determination of 5-Methoxy-N,N-Diisopropyltryptamine and its Metabolites in Urine Using Liquid Chromatography–Electrospray lonization–Tandem Mass Spectrometry. Journal of Forensic Sciences, 56: 1044–1048. doi: 10.1111/j.1556-4029.2011.01753.x
- Issue published online: 5 JUL 2011
- Article first published online: 21 MAR 2011
- Received 25 Mar. 2010; and in revised form 28 May 2010; accepted 13 June 2010.
- forensic science;
Abstract: 5-Methoxy-N,N-diisopropyltryptamine (5-MeO-DIPT) is a designer hallucinogen derived from tryptamine and is reportedly abused and involved in criminal activities. For the detection of 5-MeO-DIPT use, a liquid chromatography–tandem mass spectrometric method for 5-MeO-DIPT and its metabolites, 5-hydroxy-N,N-diisopropyltryptamine (5-OH-DIPT) and 5-methoxy-N,N-isopropyltryptamine (5-MeO-IPT) was developed and validated in rat urine. The urine samples were pretreated by protein precipitation with acetonitrile and introduced into a BDS HYPERSIL C18 column (50 × 2.0 mm, 5 μm) for chromatographic separation. Mobile phases consisted of methanol, water, and 1% formic acid, and gradient elution was used at a flow rate of 0.2 mL/min. For the MS detection, multiple-reaction monitoring analysis was adopted. The linear range was 0.01–10 μg/mL, and the lower limit of quantification was 10 ng/mL for all analytes. The intra- and interday accuracies and precisions met the criteria (<15%). The developed method was successfully applied to the drug-treated rat urine.