Presented in part at the 19th International Symposium on Human Identification, October 13–16, 2008, in Hollywood, CA.
Developmental Validation of the SPERM HY-LITERTM Kit for the Identification of Human Spermatozoa in Forensic Samples
Article first published online: 19 MAY 2011
© 2011 American Academy of Forensic Sciences
Journal of Forensic Sciences
Volume 56, Issue 4, pages 853–865, July 2011
How to Cite
Miller, K. W. P., Old, J., Fischer, B. R., Schweers, B., Stipinaite, S. and Reich, K. (2011), Developmental Validation of the SPERM HY-LITERTM Kit for the Identification of Human Spermatozoa in Forensic Samples. Journal of Forensic Sciences, 56: 853–865. doi: 10.1111/j.1556-4029.2011.01796.x
The SPERM HY-LITER™ kit was developed and is manufactured by Independent Forensics of Illinois (IFI), a company engaged in the delivery of products and services to the forensic community. The Forensic Biotechnology Institute of California (FBIC) is a nonprofit ancillary unit of the College of Science and Mathematics at the California State University, Fresno. IRB permission was obtained for all samples reported in this study and taken by personnel at the California State University, Fresno, for studies conducted by University personnel at the University. Samples collected by personnel at Independent Forensics of Illinois (IFI) and tested at IFI by IFI personnel were not subject to IRB regulation.
- Issue published online: 5 JUL 2011
- Article first published online: 19 MAY 2011
- Received 5 Feb. 2010; and in revised form 28 April 2010; accepted 22 May 2010.
- forensic science;
- forensic serology;
- SPERM HY-LITER™;
- sexual assault;
- developmental validation
Abstract: With sexual assault evidence, the visualization of spermatozoa confirms that ejaculation has occurred. However, microscopic examination of spermatozoa is a laborious process and can sometimes result in sperm cells being overlooked. Here, we present the developmental validation of the SPERM HY-LITER™ kit, which contains a human sperm–specific mouse monoclonal antibody coupled to a fluorescent Alexa 488 dye. The kit was tested using samples of human semen, saliva, blood, and urine, various animal semen extracts, sexual lubricants, and a commercially available spermicidal film. Postcoital vaginal swabs, degraded semen samples, and samples prepared with sample fixation techniques that deviated from the kit-provided protocol were also tested. In each case, the SPERM HY-LITER™ kit was demonstrated to bind only to human sperm cell heads. Limitations to this fluorescent staining procedure include nonspecific staining and increased background fluorescence with extreme heat fixation in some samples.