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Performance Evaluation and Optimization of Multiplex PCRs for the Highly Discriminating OSU 10-Locus Set Y-STRs
Article first published online: 21 SEP 2011
© 2011 American Academy of Forensic Sciences
Journal of Forensic Sciences
Volume 57, Issue 1, pages 52–59, January 2012
How to Cite
Hanson, E., Maybruck, J. L., Ballantyne, J. and Fuerst, P. A. (2012), Performance Evaluation and Optimization of Multiplex PCRs for the Highly Discriminating OSU 10-Locus Set Y-STRs. Journal of Forensic Sciences, 57: 52–59. doi: 10.1111/j.1556-4029.2011.01910.x
This study was supported by a research award from The Ohio State University. In addition, portions of this work were supported under Award number 2005-MU-MU-K044 from the Office of Justice Programs, National Institute of Justice, Department of Justice.
- Issue published online: 4 JAN 2012
- Article first published online: 21 SEP 2011
- Received 7 March 2010; and in revised form 28 Oct. 2010; accepted 21 Nov. 2010.
- forensic science;
- short tandem repeats;
Abstract: In a previous study, a new set of Y-chromosome short tandem repeats, the OSU 10-locus set (MPM1 and MPM2), was shown to have a higher discrimination power when evaluated against the 10 SWGDAM loci on a common population panel. Here, we describe the optimization of the multiplex reactions using dye-labeled primers followed by performance evaluations. The loci exhibited high precision, human male specificity, reliability in different body fluids, high sensitivity, stability, and the ability to amplify nonprobative casework and mixture samples. Stutter for the all of the loci, with the exception of the highly polymorphic locus DYS688, was similar to that observed for autosomal loci. The results of the performance evaluations reinforced the utility of these loci.